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Th2 differentiation in a time course experiment and at single generation resolution.


Mouse splenic naïve T cells were activated (with anti-CD3/CD28)and polarized towards the Th2 subtype with IL4 . Cells were collected at different time points during the differentiation and, at day 3 .5,were collected according to number of cell divisions and expression or absence of a specific differentiation marker.

E-MTAB-3543 Samples



Cell Metadata and BAM Files

Dataset Metadata

BAM Files Run Accession Number Sample Accession Number Organism Organ/Tissue Dev. Stage Cell Type Sequencer Protocol Assay Type Library Layout Ontology Term Cell Cycle Phase
ERR873424 ERS717104 Mus musculus Mediastinal Lymph Nodes Adult T helper cells subtype 2 Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000546 [T-helper 2 cell] S
ERR873425 ERS717109 Mus musculus Mediastinal Lymph Nodes Adult T helper cells subtype 2 Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000546 [T-helper 2 cell] G2.M
ERR873426 ERS717119 Mus musculus Mediastinal Lymph Nodes Adult Naive T cells activated Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000898 [naive T cell] G2
ERR873427 ERS717103 Mus musculus Mediastinal Lymph Nodes Adult Naive T cells activated Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000898 [naive T cell] S
ERR873428 ERS717112 Mus musculus Mediastinal Lymph Nodes Adult T helper cells subtype 2 Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000546 [T-helper 2 cell] M.G1
ERR873429 ERS717111 Mus musculus Mediastinal Lymph Nodes Adult T helper cells subtype 2 Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000546 [T-helper 2 cell] M.G1
ERR873430 ERS717108 Mus musculus Mediastinal Lymph Nodes Adult T helper cells subtype 2 Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000546 [T-helper 2 cell] G1.S
ERR873431 ERS717102 Mus musculus Mediastinal Lymph Nodes Adult Naive T cells activated Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000898 [naive T cell] G2
ERR873432 ERS717105 Mus musculus Mediastinal Lymph Nodes Adult T helper cells subtype 2 Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000546 [T-helper 2 cell] S
ERR873433 ERS717107 Mus musculus Mediastinal Lymph Nodes Adult Naive T cells activated Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000898 [naive T cell] G2
ERR873434 ERS717114 Mus musculus Mediastinal Lymph Nodes Adult Naive T cells activated Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000898 [naive T cell] M.G1
ERR873435 ERS717115 Mus musculus Mediastinal Lymph Nodes Adult T helper cells subtype 2 Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000546 [T-helper 2 cell] M.G1
ERR873436 ERS717110 Mus musculus Mediastinal Lymph Nodes Adult Naive T cells activated Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000898 [naive T cell] G2
ERR873437 ERS717117 Mus musculus Mediastinal Lymph Nodes Adult T helper cells subtype 2 Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000546 [T-helper 2 cell] S
ERR873438 ERS717120 Mus musculus Mediastinal Lymph Nodes Adult Naive T cells activated Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000898 [naive T cell] G2
ERR873439 ERS717101 Mus musculus Mediastinal Lymph Nodes Adult T helper cells subtype 2 Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000546 [T-helper 2 cell] S
ERR873440 ERS717106 Mus musculus Mediastinal Lymph Nodes Adult T helper cells subtype 2 Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000546 [T-helper 2 cell] S
ERR873441 ERS717116 Mus musculus Mediastinal Lymph Nodes Adult T helper cells subtype 2 Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000546 [T-helper 2 cell] G2.M
ERR873442 ERS717118 Mus musculus Mediastinal Lymph Nodes Adult Naive T cells activated Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000898 [naive T cell] G2
ERR873443 ERS717113 Mus musculus Mediastinal Lymph Nodes Adult T helper cells subtype 2 Illumina HiSeq 2000 SMARTer RNA-Seq Single-End CL:0000546 [T-helper 2 cell] M.G1

FASTQC Report

Bulk Download

FASTQC File ID Cell Accession Number FASTQC Report Link No. Sequence Tags
ERR873424 ERR873424 Preview 11589494
ERR873425 ERR873425 Preview 14132273
ERR873426 ERR873426 Preview 75815085
ERR873427 ERR873427 Preview 52327404
ERR873428 ERR873428 Preview 16329069
ERR873429 ERR873429 Preview 15190666
ERR873430 ERR873430 Preview 16207558
ERR873431 ERR873431 Preview 80379572
ERR873432 ERR873432 Preview 16216971
ERR873433 ERR873433 Preview 79328104
ERR873434 ERR873434 Preview 65905457
ERR873435 ERR873435 Preview 13883633
ERR873436 ERR873436 Preview 72610535
ERR873437 ERR873437 Preview 13904942
ERR873438 ERR873438 Preview 71285493
ERR873439 ERR873439 Preview 14827849
ERR873440 ERR873440 Preview 14343217
ERR873441 ERR873441 Preview 13328110
ERR873442 ERR873442 Preview 80445956
ERR873443 ERR873443 Preview 14167351

Organism : Mus musculus

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File Size : 46M

Mouse Cell Data

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tSNE image.
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PCA image.
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Gene Body Coverage image.

Data Resources


Organism Run Metadata Link Data Repository Link Pubmed Record Link Author Provided Files DAVID Annotation Chart Link DAVID Annotation Table Link Gene Body Coverage Matrix Link PCA Matrix Link Krona Plot Img Link Unmapped Reads Link Expression Correlation
Mus musculus