FastQCFastQC Report
Thu 26 May 2016
ERR873442.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873442.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80445956
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT29375923.65163414802355Illumina Paired End PCR Primer 2 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTT3250150.028.13449730
CGGTTCA3362150.028.061911
AATGCCG3375950.027.77652713
CGAGATC3352050.027.63387318
GATCGGA3360200.027.62337921
GAGCGGT3319200.027.56622929
TGCCGAG3404650.027.49967815
TCGGAAG3370050.027.4821823
GAGATCG3376100.027.46357519
AGATCGG3383850.027.41442720
GCCGAGA3415950.027.36789716
ATGCCGA3429200.027.36231814
AAGAGCG3353150.027.3521727
CCGAGAT3389850.027.33364117
GAATGCC3461200.027.17463712
ATCGGAA3407500.027.1634122
CGGAAGA3401150.027.13166224
AGAGCGG3381600.027.12783228
AGGAATG3526900.026.83376510
GGTTCAG3527350.026.8150622
Produced by FastQC (version 0.11.5)