FastQCFastQC Report
Thu 26 May 2016
ERR873438.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873438.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71285493
Sequences flagged as poor quality0
Sequence length36
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT52659787.387166418278119Illumina Paired End PCR Primer 2 (96% over 25bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCA1313400.18424506091302476Illumina Paired End PCR Primer 2 (100% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA5985600.028.336581
AGCGGTT5766300.028.23890530
AATGCCG6004800.028.00660313
CGAGATC5987400.027.903418
TGCCGAG6027100.027.88784415
ATGCCGA6038750.027.84964814
TCGGAAG5966600.027.8336923
CCGAGAT6012100.027.83266817
GAGATCG6010650.027.8282719
GATCGGA5999850.027.80964321
AGATCGG6011800.027.8056220
GCCGAGA6041000.027.80056616
GAGCGGT5880650.027.77722529
GAATGCC6075100.027.73189212
AAGAGCG5910250.027.62569427
CGGAAGA5976850.027.61630624
AGAGCGG5922250.027.61398128
GTTCAGC6142450.027.6093983
GGTTCAG6154150.027.6045822
GGAATGC6119850.027.58449411
Produced by FastQC (version 0.11.5)