FastQCFastQC Report
Thu 26 May 2016
ERR873432.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873432.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16216971
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28100.030.91473244
TCGTATG29800.029.22494143
CTCGTAT33050.026.28453842
ACACGTC33800.026.2232113
AGTCACG34000.025.61480128
GTCACGA34750.024.93538929
ACGATCA34800.024.83636532
TCACGAT35400.024.4154130
GATCGGA34500.024.3082451
TCTCGTA36500.023.86036141
ATCGGAA36850.023.0504472
CGATCAG38100.022.68518333
CACACGT40500.022.05231712
ACGTCTG40050.021.85623615
CGTCTGA39950.021.74504916
ATCTCGT41500.021.19759840
CACGATC41750.021.01798631
CACGTCT43200.020.16085414
GCACACG46500.019.15960711
GATCTCG46050.019.00763939
Produced by FastQC (version 0.11.5)