FastQCFastQC Report
Thu 26 May 2016
ERR873434.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873434.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65905457
Sequences flagged as poor quality0
Sequence length36
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT1009086715.311125147042073Illumina Paired End PCR Primer 2 (96% over 25bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCA2638040.40027641413669285Illumina Paired End PCR Primer 2 (100% over 22bp)
CGGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGT724600.10994537220188004Illumina Paired End PCR Primer 2 (95% over 24bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAA674850.10239668014137282Illumina Paired End PCR Primer 2 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGCAG11221100.028.5381955
CGGTTCA11211850.028.5312271
AGCGGTT11006300.028.40980530
TCGGAAG11204100.028.18927823
GGTTCAG11410700.028.1297992
AATGCCG11319950.028.12167413
GTTCAGC11410400.028.1044333
AGATCGG11268250.028.0993520
CGAGATC11269800.028.08724818
GAGATCG11291150.028.06143619
ATGCCGA11327900.028.06039814
GATCGGA11293050.028.05250221
AGGAATG11406100.028.04166610
GGAATGC11395150.028.01110311
GCCGAGA11336950.028.00581216
ATCGGAA11283050.028.00306722
TTCAGCA11443350.027.9993954
TGCCGAG11353500.027.99365415
CGGAAGA11229050.027.98861124
GAATGCC11390300.027.98445312
Produced by FastQC (version 0.11.5)