FastQCFastQC Report
Thu 26 May 2016
ERR873436.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873436.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72610535
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT5222790.7192881859361041Illumina Paired End PCR Primer 2 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTT638250.025.9665830
AATGCCG666950.025.69473513
GATCGGA659000.025.41177621
CGAGATC660600.025.3485718
CGGTTCA684100.025.3343721
TGCCGAG692900.024.75124715
GAGCGGT670200.024.7264229
GAGATCG678000.024.69360719
AGATCGG690400.024.24792320
TCGGAAG697150.024.03965423
GCCGAGA713500.024.01561516
AAGAGCG693500.023.99942627
CCGAGAT701950.023.90448617
ATGCCGA740250.023.59054814
CGGAAGA713450.023.41266324
ATCGGAA715150.023.39474922
AGAGCGG716750.023.2713928
GAATGCC749750.022.99508312
AGGAATG808400.021.60685510
CAGGAAT821400.021.3196549
Produced by FastQC (version 0.11.5)