FastQCFastQC Report
Thu 26 May 2016
ERR873429.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873429.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15190666
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAT15000.010.8886661
CTCGAAT20050.010.3477921
CGGTATT14150.09.8233072
CGACGAT15200.09.4385221
CTCGATT11950.09.4196881
CTCGTAT12450.09.3959511
TAACGCG11700.09.2402522
TGCGCGA12800.09.1082819
CGGCGAT10450.09.0821021
AACGCGT11850.08.9370973
CGGGAAT15350.08.9149231
CTAACGC13350.08.7625161
CGCAGAT24450.08.756431
ACGCGTA6301.8189894E-128.72715733
CTCGGAT28950.08.6151751
CTCGTTT27350.08.5542671
CGGAAAT15500.08.5438541
CGCGAAT4255.166403E-78.3093171
TCGAATG12250.08.2850532
CTCGGTT21950.08.2454351
Produced by FastQC (version 0.11.5)