FastQCFastQC Report
Thu 26 May 2016
ERR873425.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873425.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14132273
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG30350.033.3320639
TCGTATG31100.033.16465443
CGTATGC32100.031.99446544
CTCGTAT32500.031.66835642
CGATGTA32550.031.48480634
GATCGGA32050.029.7509731
TCTCGTA34250.029.66500541
ACACGTC34950.029.63884713
ACCGATG36150.028.53189532
CACCGAT36650.028.32266631
CCGATGT36500.028.25830533
ACGTCTG38850.026.77664415
CGTCTGA39150.026.51425216
TCACCGA39500.026.2791330
ATCTCGT39600.025.65723240
ATCGGAA37650.025.4952182
CACGTCT41050.025.44884514
CACACGT41850.024.96775412
GTCACCG42500.024.42413129
GCACACG43000.024.24902711
Produced by FastQC (version 0.11.5)