FastQCFastQC Report
Thu 26 May 2016
ERR873441.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873441.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13328110
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG23600.030.84634443
CGTATGC24550.029.652744
CTCGTAT25650.028.29530142
ACACGTC27400.027.53313813
GATCGGA24350.027.183391
TCTCGTA27750.026.0747841
ACGTCTG31000.024.33565115
CGTCTGA31350.024.06278616
ATCTCGT31300.023.25794840
CACACGT33250.022.75852612
CACGTCT33900.022.38368414
TTAGGCA34100.021.8640934
ATCGGAA31200.021.3517762
GCACACG36950.020.53920611
TAGGCAT36500.020.36619635
ACTTAGG37400.020.05251532
CTTAGGC37300.019.87042433
CACTTAG38600.019.8279631
TCACTTA39000.019.28624230
CATCTCG40600.018.03872939
Produced by FastQC (version 0.11.5)