FastQCFastQC Report
Thu 26 May 2016
ERR873427.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873427.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52327404
Sequences flagged as poor quality0
Sequence length36
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT3612520.6903686641898001Illumina Paired End PCR Primer 2 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCCG450150.025.63150613
AGCGGTT438000.025.62042230
GATCGGA443700.025.6166321
AGATCGG447850.025.45096820
CGAGATC452550.025.24538818
CGGTTCA464600.024.9843081
GAGATCG460250.024.84008819
GAGCGGT452800.024.83261929
TGCCGAG474400.024.38299415
CCGAGAT473150.024.22867817
GCCGAGA478000.024.17739116
ATGCCGA482550.024.1607514
TCGGAAG479450.023.70193723
AAGAGCG481450.023.4699727
AGAGCGG483850.023.40326328
GAATGCC501650.023.08984812
CGGAAGA491850.023.02817524
ATCGGAA494350.022.93900322
AGGAATG546550.021.46193910
GGAATGC541450.021.42030111
Produced by FastQC (version 0.11.5)