FastQCFastQC Report
Thu 26 May 2016
ERR873433.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873433.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79328104
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT45053005.679323937957726Illumina Paired End PCR Primer 2 (96% over 25bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCA1158710.14606551040221508Illumina Paired End PCR Primer 2 (100% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA5099950.028.2424221
AGCGGTT4955250.028.1880330
AATGCCG5117450.027.9264313
CGAGATC5100400.027.79038618
GATCGGA5103150.027.78601821
TGCCGAG5134750.027.771515
TCGGAAG5102450.027.7165223
GAGATCG5121350.027.69616119
GAGCGGT5047100.027.67965129
AGATCGG5121400.027.6727420
ATGCCGA5172750.027.6218914
GCCGAGA5160050.027.60540816
CCGAGAT5145200.027.58308817
AAGAGCG5070400.027.57965927
GAATGCC5200650.027.52635212
AGAGCGG5102200.027.42952228
CGGAAGA5134500.027.42430324
ATCGGAA5161900.027.40457522
AGGAATG5260200.027.33415810
GGTTCAG5280650.027.3126812
Produced by FastQC (version 0.11.5)