FastQCFastQC Report
Thu 26 May 2016
ERR873443.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873443.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14167351
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAT15550.019.65828537
TATCTCG15200.019.53221141
CCGTATC16300.018.88868338
CGTATCT17800.016.9262939
CTCGTAT19750.016.36859744
TTCCGTA19000.016.32024836
ACACGTC18800.016.02663413
GTTCCGT21450.014.45616235
GATCGGA20350.014.3186841
TCTCGTA22200.013.9677843
ATCGGAA21100.013.8061562
AGTTCCG23700.013.26937434
CGTCTGA23850.013.09389416
ACGTCTG24300.012.76122915
CACACGT25250.012.63195412
ATCTCGT26600.011.57464342
CACGTCT27550.011.33564814
GCACACG28750.011.24725311
CCGGGAT30300.010.0537951
CGGGAAT17000.09.3492581
Produced by FastQC (version 0.11.5)