FastQCFastQC Report
Thu 26 May 2016
ERR873430.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873430.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16207558
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG49050.035.69148643
CGTATGC48800.035.6039244
AATCTCG53550.032.7331739
ACACGTC53800.032.5827713
CTCGTAT53850.032.46922742
GATCGGA50700.031.7243211
TCTCGTA55300.031.45868341
ATCGGAA53400.030.1548772
ACGTCTG59800.029.35064715
CGTCTGA60350.029.2639416
CACACGT60500.029.16025412
ATCTCGT60950.028.6507540
CACGTCT62350.028.1148114
GTAATCT64500.027.07376337
GCACACG66650.026.7667511
TAATCTC72450.024.1636538
TTGTAAT77650.023.11194635
TGTAATC78600.022.38490736
CTTGTAA81800.021.77807834
TCGGAAG75600.021.4749413
Produced by FastQC (version 0.11.5)