FastQCFastQC Report
Thu 26 May 2016
ERR873435.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873435.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13883633
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAT11900.011.3146211
CCGGAAT15850.09.8874931
CGGCGAT10150.09.1335661
CGGGAAT17000.08.6992731
CTCGTAT12700.08.48514144
CCGGGAT27800.08.4162421
TTGCGCG5802.4556357E-108.3436028
CGGCAAT11250.08.2405061
CGCAAAT7600.08.1320791
CCCGATT8700.08.1187251
CCGCAAT8200.08.0754091
CGCGATT3851.03298535E-58.0264671
CGCCAAT11550.08.0264661
CCGAGAT23900.07.9425081
CGCCAAC17000.07.92023371
CGATCTA5302.806155E-87.91076854
CTCGGAT25800.07.87091541
CCGGCAT18350.07.81869751
CGTCGAT5151.4467696E-77.7147591
CGCGCTA4905.689708E-77.63178069
Produced by FastQC (version 0.11.5)