FastQCFastQC Report
Thu 26 May 2016
ERR873440.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873440.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14343217
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC90350.039.94544
GATCGGA88200.039.404171
TCGTATG92000.039.22859643
CTCGTAT92100.039.18600542
ACACGTC96750.037.87291713
TCTCGTA96000.037.6857141
ATCGGAA94400.036.9228632
CGTCTGA99650.036.7912716
ACGTCTG101550.036.10404615
ATCTCGT100600.035.962540
CACACGT102350.035.95754612
CACGTCT104250.035.2748314
GATCTCG103250.035.06079539
GCACACG106350.034.6051211
AGTGATC111350.032.66824336
TCGGAAG108150.032.269353
CGGAAGA123150.030.3807624
GTCACAC120950.030.20259329
GTGATCT121850.029.74487737
GTCTGAA131300.028.10695517
Produced by FastQC (version 0.11.5)