FastQCFastQC Report
Thu 26 May 2016
ERR873431.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873431.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80379572
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT18587372.3124494865436707Illumina Paired End PCR Primer 2 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA2747350.028.0824361
AATGCCG2749150.027.7865713
CGAGATC2639650.027.51516218
TGCCGAG2774100.027.48603815
GATCGGA2628450.027.46793621
AGCGGTT2124150.027.41273730
ATGCCGA2790650.027.37557214
GAGATCG2661400.027.31565319
GCCGAGA2785100.027.26332916
TCGGAAG2519700.027.24071323
AGATCGG2660750.027.20951720
CCGAGAT2726350.027.1841617
GAGCGGT2185400.027.07513429
GAATGCC2836050.027.00010512
AAGAGCG2220050.026.88306427
ATCGGAA2678450.026.87123722
CGGAAGA2356700.026.70638324
AGAGCGG2252200.026.56263728
AGGAATG2912200.026.51446310
GTTCAGC2919200.026.4106393
Produced by FastQC (version 0.11.5)