FastQCFastQC Report
Thu 26 May 2016
ERR873426.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873426.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75815085
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTT17279952.2792231915323975Illumina Paired End PCR Primer 2 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA2032250.027.7964651
AGCGGTT1948500.027.77892130
AATGCCG2024950.027.66543613
GATCGGA2006550.027.38973621
CGAGATC2008500.027.3431118
GAGCGGT1992250.027.16889429
TGCCGAG2060650.027.15471515
GAGATCG2025400.027.14753519
ATGCCGA2075150.027.05133414
AGATCGG2033400.027.02592520
TCGGAAG2032300.027.00106223
CCGAGAT2037650.026.96077717
GCCGAGA2074400.026.939316
ATCGGAA2042250.026.85702522
AAGAGCG2023800.026.83820727
CGGAAGA2048100.026.66973524
AGAGCGG2039000.026.65910728
GAATGCC2113150.026.50144212
GGAATGC2159800.025.97902111
AGGAATG2180400.025.84705510
Produced by FastQC (version 0.11.5)