FastQCFastQC Report
Thu 26 May 2016
ERR873439.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873439.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14827849
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC12300.015.19802644
TCGTATG13600.014.06864443
ACACGTC15550.012.30482413
CTCGTAT16900.010.80098242
CCGCAAT9050.09.9989281
GATCGGA17300.09.82342151
AATCTCG19750.09.68780839
ACGTCTG20350.09.61860615
CCGGTAT14200.09.4811211
CGGCAAT12350.09.4716821
CGTCTGA22750.09.08713716
TCTCGTA20250.09.01415341
CACGTCT21900.08.93783714
ATCGGAA19200.08.8489162
CCGGCAT17050.08.8024331
CCGGGAT35550.08.6296541
CACACGT24250.08.52680112
CGACGAT14500.08.5238971
CGGCGAT12150.08.1743721
CGATCTA4652.3493885E-78.0666864
Produced by FastQC (version 0.11.5)