FastQCFastQC Report
Thu 26 May 2016
ERR873428.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873428.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16329069
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAT16200.011.5779571
CGGTATT15200.09.4336352
CGACGAT15250.09.2605791
CTCGAAT21800.09.0086881
CTCGTAT14500.08.6743091
CGGGAAT18350.08.6581361
TGCGCGA14100.08.580539
CCGGAAT19350.08.4387631
CTCGGAT30900.08.4265831
TCGAATG14450.08.3966132
CCGCAAT10450.08.2352411
CCGAGAT28750.08.2124731
CTAACGC15100.08.18351
CGCCACT24900.08.1529831
TAACGCG13700.08.0511652
CGCCAAC18850.07.9602291
CGGAAAT17000.07.917881
CCCGGTT19300.07.8889611
CGAGAAT19100.07.8560381
CTCGATT13550.07.8168171
Produced by FastQC (version 0.11.5)