FastQCFastQC Report
Thu 26 May 2016
ERR873424.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873424.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11589494
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC26050.032.50478444
ACGGCTA27450.031.16706732
GATCGGA26500.030.6384071
TCGTATG28700.029.58010543
ACACGTC29600.029.05272513
AGTCACG29950.028.78577628
CTCGTAT30250.027.77360742
ATCGGAA29600.027.6468072
ACGTCTG32550.026.62262215
TCTCGTA32100.026.44689641
CGTCTGA33650.025.81725716
CACACGT34400.025.32235712
CACGTCT34750.024.93694314
ATCTCGT34600.024.40886340
TCACGGC35550.024.3750430
GTCACGG36800.023.48731629
CGGCTAC36450.023.47149733
CATCTCG38650.022.02185639
GCACACG41800.021.31335311
CACGGCT42350.020.30542431
Produced by FastQC (version 0.11.5)