FastQCFastQC Report
Thu 26 May 2016
ERR873437.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR873437.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13904942
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG54000.037.87613343
TATCTCG54200.037.6146439
CGTATGC54700.037.27081744
CTCGTAT55300.036.7868942
CGCCAAT55850.036.5426233
GATCGGA50600.036.4605371
TCTCGTA57700.035.37110541
ACACGTC58250.035.15191313
AGTCACG58600.035.12797528
GTCACGC58650.034.8730429
CGTCTGA60800.033.78503816
ACGTCTG61300.033.6180715
ACGCCAA61400.033.20368632
CACACGT62900.032.6638912
ATCGGAA57100.032.4191972
CACGTCT65000.031.6369814
ATCTCGT64700.031.51025640
GCACACG67350.030.60401511
ATATCTC67600.030.41864238
CCAATAT67500.030.23563835
Produced by FastQC (version 0.11.5)