Single-cell sequencing of peripheral blood and tumor-infiltrating immune cells in renal clear cell carcinoma [5' RNA expression sequencing]

We generated a comprehensive immune profile using single-cell RNA-seq data from 25,000 immune cells from three renal cell carcinoma along with matched peripheral blood. In addition, we performed VDJ sequencing on the isolated single T cells. In order to investigate heterogeneity and dynamics of cancer-associated immune cells, we performed single-cell sequencing on peripheral and tumor-infiltrating immune cells in three renal clear cell carcinoma patients. We chose renal clear cell carcinoma tumors based on the responsive of these tumors to immune checkpoint blockade in the context of low mutational loads, which implies a strong influence from the tumor microenvironment. Using the 10x Genomic 5 expression platform a total of 25,672 immune cells were isolated and passed filtering for quality control, with 13,433 cells from peripheral blood and 12,239 tumor-infiltrating cells. In addition, we used the Chromium Single Cell V(D)J kit to enrich for T cell receptor sequences for nearly 10,000 T cells in with accompanying expression information, allowing for the investigation of clonality and transcriptional phenotypic diversity.

CellRanger Metadata

Preview	Accession	Estimated No. of Cells	Mean Reads per Cell	Median Genes per Cell	No. of Reads	% Valid Barcodes	% sequencing Saturation	% Q30 Bases in Barcode	% Q30 Bases in UMI	% Reads Mapped to Genome	% Reads Mapped Confidently to Genome	% Reads Mapped Confidently to Intergenic Regions	% Reads Mapped Confidently to Intronic Regions	% Reads Mapped Confidently to Exonic Regions	% Reads Mapped Confidently to Transcriptome	% Reads Mapped Antisense to Gene	% Fraction Reads in Cells	Total Genes Detected	Median UMI Counts Per Cell
Preview	SRR8088290	6542	55230	1636	361314203	91.2	83.8	98.3	98.4	97.1	92.0	6.9	7.2	77.8	78.4	5.9	96.8	1636	25433
Preview	SRR8088291	6244	55736	1711	348014794	92.0	84.8	97.8	97.8	96.6	92.2	6.7	7.1	78.4	79.4	5.3	94.8	1711	24423
Preview	SRR8088292	3833	87681	1491	336081557	90.3	89.5	97.7	97.7	96.3	89.0	8.6	9.4	71.1	74.2	5.4	81.3	1491	23584
Preview	SRR8088293	6994	47583	1576	332793494	90.6	82.5	98.4	98.5	96.3	88.8	7.2	8.0	73.7	75.9	4.9	94.4	1576	23305
Preview	SRR8088294	5601	62905	1332	352329790	90.8	89.6	97.9	97.9	96.5	89.3	7.2	8.1	74.0	76.8	4.5	94.4	1332	22696
Preview	SRR8088295	2643	128756	1471	340302115	91.0	94.4	97.7	97.7	96.2	89.2	6.6	7.5	75.2	77.4	4.5	91.8	1471	20809

CellxGene data viewer

Select Run

Download Anndata Object (.h5ad)

CAGE Transcription Start Site (CTSS) File Download

The CTSS positions were taken from dataset BAM files, downloadable here.

Top 10 Marker Genes per Cluster

Seurat Cluster	Gene Symbol	P Value	Average Log	Pct 1	Pct 2	Adjusted P Value
0	CD8A	1.19E-93	2.8587132	None	None	2.57E-89
0	RPL28	5.50E-54	0.41761374	None	None	2.37E-50
0	CD3D	1.77E-40	1.3597426	None	None	5.43E-37
0	ITM2C	1.13E-57	2.5000272	None	None	6.09E-54
0	GZMA	6.62E-36	1.3142344	None	None	1.78E-32
0	CD8B	2.89E-80	2.8199399	None	None	2.07E-76
0	CXCR4	3.78E-33	1.0549213	None	None	8.15E-30
0	GZMK	4.82E-89	3.1574037	None	None	5.19E-85
0	APOBEC3G	7.29E-31	1.1466389	None	None	1.43E-27
0	TMSB4X	5.57E-44	0.43165794	None	None	2.00E-40
1	IL7R	1.67E-67	2.6165576	None	None	1.20E-63
1	SLC2A3	1.68E-41	1.378429	None	None	2.41E-38
1	TPT1	2.08E-66	0.6149006	None	None	1.12E-62
1	VIM	1.16E-80	1.495651	None	None	1.25E-76
1	CD52	5.37E-52	1.3595276	None	None	1.29E-48
1	CDKN1A	1.49E-43	1.8643354	None	None	2.67E-40
1	ICOS	4.19E-41	1.9290165	None	None	5.64E-38
1	GPR183	1.45E-49	2.3875952	None	None	3.13E-46
1	RPLP1	7.94E-42	0.4735864	None	None	1.22E-38
1	RPL28	6.62E-63	0.49178523	None	None	2.85E-59
2	CMC1	2.37E-77	2.6757371	None	None	1.70E-73
2	CD247	8.33E-40	1.4378783	None	None	1.79E-36
2	TYROBP	2.94E-79	3.0832088	None	None	3.17E-75
2	KLRD1	8.46E-92	2.9679558	None	None	1.82E-87
2	KLRB1	2.62E-71	2.822737	None	None	1.41E-67
2	KLRF1	2.77E-51	3.235398	None	None	7.46E-48
2	NKG7	5.02E-37	2.114852	None	None	8.32E-34
2	KLRC2	2.75E-58	3.458914	None	None	8.46E-55
2	KLRK1	2.40E-42	1.8411286	None	None	5.75E-39
2	TRDC	3.50E-37	2.7720666	None	None	6.29E-34
3	PRF1	3.48E-152	3.6729715	None	None	7.50E-148
3	FCGR3A	7.14E-140	4.2721705	None	None	3.08E-136
3	NKG7	5.51E-143	3.3138227	None	None	2.97E-139
3	CD247	3.59E-116	2.530787	None	None	9.66E-113
3	FGFBP2	1.21E-119	4.099715	None	None	3.71E-116
3	KLRD1	1.86E-107	3.2563198	None	None	4.44E-104
3	GNLY	4.20E-148	4.916917	None	None	3.02E-144
3	SPON2	1.73E-132	4.6161656	None	None	6.22E-129
3	MYOM2	9.35E-98	4.990933	None	None	2.01E-94
3	GZMB	3.19E-149	4.118154	None	None	3.44E-145
4	HLA-C	7.91E-37	0.60495585	None	None	1.89E-33
4	GZMH	5.61E-84	2.774457	None	None	1.21E-79
4	GZMB	5.66E-56	2.4718456	None	None	2.44E-52
4	CD8A	6.03E-81	3.0618806	None	None	6.49E-77
4	FGFBP2	5.30E-50	2.6435487	None	None	1.90E-46
4	NKG7	1.49E-71	2.60497	None	None	1.07E-67
4	KLRD1	9.71E-40	2.0356977	None	None	2.61E-36
4	CD8B	2.77E-61	2.7477021	None	None	1.49E-57
4	CD3G	4.16E-31	1.5327085	None	None	8.96E-28
4	KLRC4	7.46E-49	2.6813235	None	None	2.30E-45
5	B2M	2.47E-36	0.4523941	None	None	1.33E-32
5	LYAR	2.10E-23	1.5687153	None	None	4.11E-20
5	IL7R	8.24E-30	2.084955	None	None	2.54E-26
5	HLA-C	5.08E-36	0.66153383	None	None	2.19E-32
5	S100A10	3.72E-27	1.0920494	None	None	1.00E-23
5	ANXA1	3.91E-26	1.5997603	None	None	9.36E-23
5	FGFBP2	3.42E-47	2.735704	None	None	3.68E-43
5	CD52	7.09E-32	1.2980722	None	None	2.55E-28
5	NKG7	2.54E-41	2.2611525	None	None	1.82E-37
5	GZMH	4.33E-80	2.9385083	None	None	9.33E-76
6	CD27	6.97E-109	4.1440916	None	None	7.51E-105
6	TOX	2.08E-79	3.208373	None	None	7.47E-76
6	HLA-DQA1	5.44E-67	2.7045834	None	None	1.07E-63
6	CD74	4.28E-68	2.1227221	None	None	9.23E-65
6	LYST	9.03E-90	3.0849836	None	None	6.49E-86
6	GZMK	1.82E-132	5.064838	None	None	3.93E-128
6	CST7	3.81E-76	2.0718205	None	None	1.17E-72
6	CMC1	1.25E-75	2.971285	None	None	3.36E-72
6	CD8B	2.59E-71	3.3858225	None	None	6.20E-68
6	CD8A	1.63E-86	3.5769196	None	None	8.79E-83
7	CD27	3.09E-65	3.0157511	None	None	5.12E-62
7	S100A4	9.33E-70	1.91282	None	None	2.23E-66
7	TNFRSF18	6.19E-68	4.102734	None	None	1.33E-64
7	CORO1B	7.59E-77	2.954491	None	None	3.27E-73
7	LTB	1.23E-70	2.9290364	None	None	3.32E-67
7	IL32	1.26E-111	2.299458	None	None	2.72E-107
7	BATF	9.33E-83	3.2072046	None	None	5.03E-79
7	TBC1D4	7.71E-71	4.5341253	None	None	2.37E-67
7	FOXP3	6.38E-108	10.805433	None	None	6.88E-104
7	CARD16	1.97E-65	2.5818732	None	None	3.54E-62
8	KIR2DL4	3.90E-92	6.49047	None	None	1.40E-88
8	HOPX	3.88E-96	3.43063	None	None	1.67E-92
8	TMIGD2	1.03E-81	4.871055	None	None	2.21E-78
8	XCL1	6.48E-92	4.3454037	None	None	2.00E-88
8	CD7	7.77E-110	3.5082643	None	None	1.67E-105
8	KLRB1	1.71E-96	4.0399847	None	None	9.23E-93
8	KRT81	1.47E-103	6.4350424	None	None	1.59E-99
8	TRDC	8.85E-88	4.946257	None	None	2.38E-84
8	KRT86	3.16E-97	5.741669	None	None	2.27E-93
8	ZNF683	2.60E-82	4.0297575	None	None	6.22E-79
9	RORA	2.15E-29	1.7347444	None	None	6.62E-26
9	ZNF683	1.04E-23	2.1412146	None	None	2.03E-20
9	B2M	3.40E-25	0.42306694	None	None	7.32E-22
9	GZMH	3.12E-58	2.8316529	None	None	3.36E-54
9	GNLY	1.82E-34	2.687875	None	None	7.85E-31
9	ANXA1	4.53E-26	1.7466844	None	None	1.08E-22
9	TRBV2	2.30E-102	7.032297	None	None	4.95E-98
9	HLA-C	3.40E-26	0.63844687	None	None	9.15E-23
9	CD52	3.39E-35	1.468536	None	None	1.83E-31
9	TRAV2	2.82E-57	5.755636	None	None	2.03E-53
10	RPL10	1.97E-90	1.0274795	None	None	7.08E-87
10	RPL13	1.19E-89	1.1609336	None	None	3.65E-86
10	RPL11	4.41E-89	1.0773872	None	None	1.06E-85
10	RPL34	3.85E-89	1.0502244	None	None	1.04E-85
10	RPS6	1.76E-88	1.2796806	None	None	3.78E-85
10	RPS8	1.82E-96	1.4294918	None	None	3.93E-92
10	RPS12	4.37E-95	1.2488374	None	None	4.70E-91
10	RPS3A	7.89E-95	1.2881902	None	None	5.66E-91
10	RPL32	1.18E-94	1.2850106	None	None	6.36E-91
10	EEF1A1	5.26E-93	0.99906784	None	None	2.27E-89
11	CD74	2.86E-77	4.1154714	None	None	3.08E-73
11	HLA-DPB1	7.22E-74	4.543598	None	None	5.19E-70
11	TYROBP	6.82E-57	4.102228	None	None	1.47E-53
11	FTL	5.49E-73	3.1924398	None	None	2.96E-69
11	CST3	1.03E-66	6.956228	None	None	3.70E-63
11	HLA-DRB1	3.73E-60	4.102569	None	None	1.15E-56
11	HLA-DPA1	9.90E-70	4.7714224	None	None	4.27E-66
11	NPC2	8.66E-59	4.152652	None	None	2.33E-55
11	HLA-DRA	4.39E-78	5.8234687	None	None	9.45E-74
11	LYZ	1.21E-58	6.117744	None	None	2.89E-55
12	RPS23	1.60E-60	1.0724887	None	None	3.46E-57
12	CD74	1.27E-72	3.6613355	None	None	1.37E-68
12	MS4A1	7.58E-60	7.9732013	None	None	1.26E-56
12	CD37	8.63E-70	2.5045464	None	None	4.65E-66
12	RPL18A	3.26E-60	1.20425	None	None	6.38E-57
12	RPS5	5.76E-60	1.383677	None	None	1.04E-56
12	HLA-DRA	5.24E-71	4.8271017	None	None	3.76E-67
12	CD79A	1.15E-72	9.40259	None	None	1.37E-68
12	RPS8	2.02E-66	1.3193978	None	None	8.70E-63
12	CD83	4.73E-64	4.1396923	None	None	1.46E-60
13	FTL	3.26E-69	3.12478	None	None	7.28E-66
13	LST1	2.99E-72	6.460263	None	None	1.29E-68
13	S100A6	7.50E-71	2.777976	None	None	2.02E-67
13	S100A8	3.38E-69	10.353324	None	None	7.28E-66
13	IFI30	3.07E-71	6.8211684	None	None	9.46E-68
13	FCN1	9.89E-76	10.16219	None	None	2.13E-71
13	S100A9	5.97E-74	10.052001	None	None	4.28E-70
13	AIF1	1.08E-71	6.379124	None	None	3.89E-68
13	CTSS	7.69E-73	4.6479344	None	None	4.14E-69
13	LYZ	8.11E-75	8.397457	None	None	8.74E-71
14	TRBV11-1	1.20E-36	8.366204	None	None	2.59E-32
14	TRGV2	4.38E-19	4.1101823	None	None	3.15E-15
14	IKZF2	1.45E-10	3.0117996	None	None	3.48E-07
14	KLRD1	2.31E-13	2.28118	None	None	9.96E-10
14	CD8A	7.23E-16	2.6398816	None	None	3.90E-12
14	KLRC2	1.13E-11	2.5274816	None	None	3.04E-08
14	TRAV38-1	3.14E-28	8.716557	None	None	3.38E-24
14	AOAH	3.57E-10	1.8251185	None	None	7.69E-07
14	CST7	1.11E-12	1.6187695	None	None	4.00E-09
14	TRGV8	1.42E-12	4.973506	None	None	4.38E-09
15	KLRC2	1.23E-10	3.553072	None	None	2.65E-06
15	LINC02446	3.46E-10	4.0447636	None	None	2.73E-06
15	KIR2DL3	9.74E-06	3.3014493	None	None	0.01166169
15	CD8A	3.93E-06	1.8819908	None	None	0.006354298
15	PARP8	2.16E-08	1.8281056	None	None	9.31E-05
15	TRBV4-2	3.79E-10	6.488491	None	None	2.73E-06
15	TRAV9-2	9.62E-08	4.808992	None	None	0.000296193
15	ZNF683	6.60E-08	2.6459794	None	None	0.000237037
15	TRAV14DV4	8.32E-10	5.4414167	None	None	4.48E-06
15	GNLY	1.94E-05	2.2904282	None	None	0.019021102
16	TUBA1B	2.87E-08	2.5586846	None	None	6.42E-05
16	PTMA	2.98E-08	0.9540778	None	None	6.42E-05
16	STMN1	2.10E-16	6.368981	None	None	4.53E-12
16	MCM7	1.82E-08	4.4914365	None	None	4.90E-05
16	DUT	1.29E-10	3.4457784	None	None	5.54E-07
16	HMGN2	1.84E-11	2.5458071	None	None	9.89E-08
16	HMGB2	1.02E-09	2.7296002	None	None	3.67E-06
16	HMGB1	1.12E-11	1.9796633	None	None	8.05E-08
16	TUBB	7.02E-14	3.4399862	None	None	7.56E-10
16	PCLAF	1.31E-09	7.678967	None	None	4.04E-06
17	HLA-DQA1	9.96E-07	3.4347825	None	None	0.004293218
17	HLA-DRB1	6.16E-05	2.4919496	None	None	0.046375216
17	CD74	7.42E-10	3.4090455	None	None	1.60E-05
17	HLA-DPB1	7.45E-06	2.7552185	None	None	0.013375036
17	RAB11FIP1	2.99E-05	2.8212225	None	None	0.034151068
17	SEC61B	2.95E-06	2.1932468	None	None	0.009080804
17	HLA-DRA	5.73E-07	3.8493285	None	None	0.003085111
17	CST3	6.24E-05	4.205586	None	None	0.046375216
17	HLA-DQB1	1.90E-08	4.1797943	None	None	0.000204608
17	GPR183	3.61E-06	3.3814793	None	None	0.009578947
18	IGHV3-48	2.30E-13	17.818605	None	None	5.83E-10
18	IGKV4-1	2.75E-13	10.159618	None	None	5.83E-10
18	IGLV6-57	2.35E-13	14.010626	None	None	5.83E-10
18	SSR4	3.13E-13	3.3498292	None	None	5.83E-10
18	JCHAIN	2.30E-13	14.0188875	None	None	5.83E-10
18	IGLV2-23	2.30E-13	14.982982	None	None	5.83E-10
18	IGLC3	2.63E-13	9.567198	None	None	5.83E-10
18	IGLC2	2.37E-13	11.671875	None	None	5.83E-10
18	IGLV1-51	2.30E-13	18.472261	None	None	5.83E-10
18	IGHA1	2.36E-13	10.911486	None	None	5.83E-10
19	SLC18A2	1.01E-06	11.96207	None	None	0.003199408
19	TPSB2	9.92E-07	36.004055	None	None	0.003199408
19	NFKBIZ	3.02E-06	3.9657052	None	None	0.006401666
19	LGALS3	3.27E-06	4.406324	None	None	0.006401666
19	HPGDS	9.92E-07	12.755974	None	None	0.003199408
19	IFITM3	2.08E-06	5.7507906	None	None	0.005290566
19	TPSAB1	9.92E-07	36.15246	None	None	0.003199408
19	LTC4S	1.04E-06	7.800258	None	None	0.003199408
19	TPSD1	9.92E-07	33.3278	None	None	0.003199408
19	GATA2	9.97E-07	12.309232	None	None	0.003199408
20	CD24	0.000547326	8.976716	None	None	0.841126564
20	MIF	0.000557458	3.9596696	None	None	0.841126564
20	ANXA4	0.00055653	6.20768	None	None	0.841126564
20	CRYAB	0.000552831	11.08684	None	None	0.841126564
20	NNMT	0.000537366	10.881002	None	None	0.841126564
20	RARRES2	0.000537366	13.175091	None	None	0.841126564
20	ADIRF	0.000536468	11.7633705	None	None	0.841126564
20	NDUFA4L2	0.000536468	11.499596	None	None	0.841126564
20	CCND1	0.000560252	8.150952	None	None	0.841126564
20	SPP1	0.000561186	9.421297	None	None	0.841126564
0	TRDV1	5.03E-149	5.2049212	None	None	1.04E-144
0	CCL5	8.38E-84	1.7475934	None	None	3.45E-80
0	TRBC1	5.45E-71	2.3444366	None	None	1.87E-67
0	FXYD2	1.98E-92	3.371689	None	None	1.36E-88
0	LAG3	3.33E-70	2.2509322	None	None	9.79E-67
0	CST7	6.25E-68	1.3879806	None	None	1.61E-64
0	TRGV8	1.79E-115	5.200185	None	None	1.84E-111
0	PMCH	5.30E-62	4.041238	None	None	1.21E-58
0	RTKN2	2.69E-90	3.894126	None	None	1.39E-86
0	GZMK	2.22E-60	2.136469	None	None	4.58E-57
1	RPS28	2.05E-93	0.70708203	None	None	3.25E-90
1	TPT1	1.14E-93	0.8184955	None	None	1.96E-90
1	RPS29	3.73E-97	0.9533093	None	None	8.54E-94
1	IL7R	2.41E-125	5.294469	None	None	2.48E-121
1	RPS18	4.35E-113	1.08265	None	None	2.24E-109
1	RPS12	8.99E-100	0.8188261	None	None	2.32E-96
1	RPS14	1.64E-94	0.7421732	None	None	3.08E-91
1	RPL39	6.12E-111	0.83440495	None	None	2.52E-107
1	RPL13	2.47E-96	0.7918664	None	None	5.10E-93
1	RPL28	1.69E-101	0.7055643	None	None	5.80E-98
2	KLRD1	3.17E-170	5.8884687	None	None	6.53E-166
2	KLRB1	2.93E-150	5.161805	None	None	8.64E-147
2	MYOM2	1.16E-121	7.9353933	None	None	1.85E-118
2	FCGR3A	3.94E-142	5.0431314	None	None	7.39E-139
2	SPON2	1.19E-150	5.44539	None	None	4.08E-147
2	GZMB	9.57E-164	4.9396663	None	None	9.87E-160
2	FGFBP2	2.69E-145	6.9124427	None	None	5.55E-142
2	KLRF1	1.17E-151	6.9599466	None	None	4.85E-148
2	GNLY	8.23E-146	5.785733	None	None	2.12E-142
2	TYROBP	7.11E-154	5.512248	None	None	3.67E-150
3	TRBC2	1.25E-84	2.0088816	None	None	5.14E-81
3	HLA-DRB1	9.41E-34	1.5662109	None	None	1.76E-30
3	CD8A	4.10E-106	3.5746958	None	None	2.12E-102
3	CD74	1.34E-48	1.6144336	None	None	3.95E-45
3	TRAV12-2	1.41E-164	7.459789	None	None	2.90E-160
3	CST7	9.05E-36	1.2156875	None	None	1.87E-32
3	TRBV20-1	3.46E-160	7.4017277	None	None	3.56E-156
3	CD3D	1.41E-39	1.2649648	None	None	3.22E-36
3	CD8B	1.83E-114	3.570796	None	None	1.26E-110
3	TRAV22	3.17E-47	5.609035	None	None	8.18E-44
4	GZMK	2.76E-28	1.8614542	None	None	1.14E-24
4	HLA-DQA1	1.87E-27	1.2658111	None	None	6.43E-24
4	ITM2A	2.67E-27	1.4623935	None	None	7.88E-24
4	HLA-DRB1	1.15E-28	1.4541706	None	None	5.92E-25
4	CD74	4.43E-27	1.2593561	None	None	1.14E-23
4	IL32	6.19E-27	1.1813821	None	None	1.42E-23
4	HLA-DPA1	2.22E-22	1.0439669	None	None	4.59E-19
4	GZMA	1.62E-43	1.3160347	None	None	1.11E-39
4	CD8A	7.56E-71	3.0120423	None	None	1.56E-66
4	CD8B	3.06E-48	2.3247714	None	None	3.16E-44
5	TRBV27	9.41E-46	6.9738126	None	None	6.47E-42
5	HLA-DRB1	7.10E-23	1.612445	None	None	1.83E-19
5	MIR155HG	3.84E-28	2.118051	None	None	1.58E-24
5	HLA-DQA1	3.15E-27	1.570564	None	None	1.08E-23
5	CD8B	1.17E-48	2.8498526	None	None	1.20E-44
5	ITM2A	3.79E-22	1.6475544	None	None	8.69E-19
5	CD8A	1.95E-55	3.2573721	None	None	4.02E-51
5	RGS1	1.08E-31	1.6014444	None	None	5.57E-28
5	CD74	6.97E-24	1.4203441	None	None	2.05E-20
5	TOX	1.34E-20	1.3854719	None	None	2.76E-17
6	TRDV1	1.69E-69	4.4375863	None	None	1.16E-65
6	CST7	5.01E-49	1.5411795	None	None	1.29E-45
6	CMC1	5.30E-67	2.926847	None	None	2.73E-63
6	CADM1	2.04E-64	3.2880013	None	None	8.42E-61
6	TRGV2	5.04E-99	5.7740235	None	None	5.20E-95
6	ADTRP	4.92E-53	3.2336936	None	None	1.69E-49
6	SNAP47	1.01E-43	2.2887201	None	None	2.07E-40
6	RTKN2	2.70E-44	3.1905751	None	None	6.19E-41
6	TRBV24-1	1.31E-103	9.222713	None	None	2.70E-99
6	PRF1	6.44E-51	2.1697352	None	None	1.90E-47
7	TIGIT	6.89E-35	2.7013485	None	None	1.09E-31
7	SLAMF1	1.13E-29	5.1521993	None	None	1.17E-26
7	SAMSN1	1.23E-34	2.3005114	None	None	1.70E-31
7	S100A4	1.17E-34	1.5251157	None	None	1.70E-31
7	AL136456.1	3.53E-42	6.38125	None	None	8.09E-39
7	BATF	6.23E-34	2.4792938	None	None	8.03E-31
7	CARD16	5.17E-37	2.2752135	None	None	9.69E-34
7	IL32	7.14E-36	1.7259804	None	None	1.23E-32
7	TBC1D4	2.76E-37	3.9934206	None	None	5.69E-34
7	FOXP3	1.72E-45	10.53366	None	None	5.06E-42
8	RPS12	4.39E-23	0.78257895	None	None	2.27E-19
8	RPL34	1.51E-20	0.69590795	None	None	3.11E-17
8	ANXA1	7.67E-21	3.2829123	None	None	2.03E-17
8	RPS18	1.51E-20	0.90979	None	None	3.11E-17
8	GZMH	3.08E-28	3.447106	None	None	6.26E-24
8	CD52	5.95E-21	2.213187	None	None	2.03E-17
8	RPL3	2.86E-24	1.1093147	None	None	1.96E-20
8	RPL14	7.34E-23	0.9387594	None	None	3.03E-19
8	GNLY	6.07E-28	4.903165	None	None	6.26E-24
8	RPL39	7.86E-21	0.74630654	None	None	2.03E-17
9	CD52	8.77E-15	2.1164992	None	None	2.26E-11
9	PARP8	2.17E-13	1.8182665	None	None	3.72E-10
9	LINC02446	2.00E-27	8.554037	None	None	4.12E-23
9	RPS18	3.23E-14	0.8959564	None	None	7.39E-11
9	XCL1	3.76E-21	4.9915085	None	None	1.55E-17
9	ZNF683	5.44E-27	7.324744	None	None	5.61E-23
9	KLRD1	2.28E-15	3.4238079	None	None	6.72E-12
9	XCL2	1.41E-22	4.391644	None	None	7.26E-19
9	LINC01871	1.31E-23	4.0254602	None	None	8.98E-20
9	RPL30	2.72E-13	0.62497574	None	None	4.32E-10
10	NPC2	2.38E-24	4.7312512	None	None	4.90E-21
10	PSAP	1.69E-23	3.2482016	None	None	2.68E-20
10	IFI30	1.78E-28	6.7048016	None	None	1.22E-24
10	TYROBP	3.43E-26	5.4186068	None	None	1.01E-22
10	FTH1	1.05E-28	3.0804482	None	None	1.08E-24
10	SAT1	3.50E-24	3.2720234	None	None	6.56E-21
10	CST3	2.80E-29	8.307303	None	None	5.77E-25
10	FTL	6.51E-24	2.613273	None	None	1.12E-20
10	LYZ	9.94E-28	8.020203	None	None	4.10E-24
10	SERPINA1	5.69E-28	8.738053	None	None	2.93E-24
11	HLA-DRB1	3.32E-13	2.293948	None	None	6.85E-09
11	ACTB	8.76E-10	0.8309032	None	None	1.50E-06
11	CD8B	2.47E-09	2.373406	None	None	3.64E-06
11	HMGN2	8.76E-11	1.8013859	None	None	3.09E-07
11	CD74	4.42E-11	1.790008	None	None	2.56E-07
11	TUBB	7.66E-10	2.0380316	None	None	1.44E-06
11	HLA-DPA1	3.37E-10	1.6734071	None	None	8.69E-07
11	HLA-DRA	4.96E-11	2.2222006	None	None	2.56E-07
11	NUCKS1	3.14E-09	1.9195594	None	None	4.32E-06
11	STMN1	9.00E-11	4.124918	None	None	3.09E-07
12	KLRB1	2.25E-14	4.2237406	None	None	1.16E-10
12	KLRK1	2.31E-09	2.1302605	None	None	4.32E-06
12	KLRD1	1.78E-14	4.211657	None	None	1.16E-10
12	MAPK1	1.98E-09	3.057204	None	None	4.08E-06
12	AREG	1.58E-15	4.653966	None	None	1.63E-11
12	TYROBP	4.87E-16	4.5654655	None	None	1.00E-11
12	CD7	2.61E-09	2.2758543	None	None	4.49E-06
12	XCL2	4.13E-10	4.208623	None	None	9.47E-07
12	KRT81	1.34E-11	8.093541	None	None	3.96E-08
12	KRT86	6.06E-12	5.7058063	None	None	2.08E-08
13	THEMIS	2.45E-05	2.0682106	None	None	0.063238225
13	RASSF4	7.86E-06	3.2018213	None	None	0.032421805
13	ZNF331	6.90E-05	1.6480713	None	None	0.142331978
13	CD8A	8.86E-07	2.8535736	None	None	0.004566293
13	TRBV9	3.04E-13	9.503804	None	None	3.13E-09
13	TRAV12-1	1.42E-14	9.530298	None	None	2.92E-10
13	INPP4B	5.29E-05	2.1565351	None	None	0.121236155
13	FXYD2	1.89E-05	2.5244644	None	None	0.055775774
13	TRBC1	1.21E-05	2.0431604	None	None	0.04153189
13	TRAV13-2	5.06E-12	8.238698	None	None	3.48E-08
14	CD83	9.28E-06	4.7951975	None	None	0.037163448
14	BANK1	2.00E-05	9.310735	None	None	0.038847197
14	RPL39	1.76E-05	1.1604788	None	None	0.038847197
14	CD74	1.08E-05	2.966309	None	None	0.037163448
14	HLA-DRA	6.56E-06	4.187017	None	None	0.033805007
14	EEF1B2	4.99E-06	1.9394693	None	None	0.033805007
14	RPS23	2.27E-06	1.3514445	None	None	0.023417897
14	RPS8	1.42E-06	1.6516064	None	None	0.023417897
14	RPL5	2.07E-05	1.2544245	None	None	0.038847197
14	EEF1A1	2.68E-05	0.8981809	None	None	0.03971606
0	GZMK	4.47E-88	3.3531203	None	None	8.75E-84
0	TOX	8.30E-52	2.46705	None	None	1.81E-48
0	TNFRSF9	1.28E-52	3.9047792	None	None	3.13E-49
0	ITM2A	9.28E-53	2.4601214	None	None	2.60E-49
0	HLA-DRB1	7.32E-59	2.3234966	None	None	2.39E-55
0	DUSP4	1.60E-59	2.4575663	None	None	6.27E-56
0	LYST	1.77E-67	2.8303115	None	None	8.67E-64
0	CD27	1.65E-70	3.072759	None	None	1.07E-66
0	CD74	4.59E-84	2.3717654	None	None	4.49E-80
0	HLA-DPA1	1.75E-51	2.1326742	None	None	3.42E-48
1	GZMM	2.47E-13	1.3943533	None	None	4.83E-10
1	RPS15A	1.19E-22	0.71340626	None	None	1.17E-18
1	GZMA	6.06E-17	1.3815374	None	None	2.37E-13
1	RPS29	1.45E-26	0.86021554	None	None	2.83E-22
1	RPL13	5.33E-16	0.61988056	None	None	1.74E-12
1	CD8A	1.41E-15	1.612694	None	None	3.45E-12
1	RPS27	4.67E-19	0.673162	None	None	2.29E-15
1	RPL23A	3.21E-14	0.70707804	None	None	6.99E-11
1	GZMK	2.82E-19	2.0856142	None	None	1.84E-15
1	CD8B	7.89E-16	1.6511674	None	None	2.21E-12
2	KLRF1	2.95E-49	6.2614794	None	None	9.64E-46
2	NKG7	6.83E-58	2.749358	None	None	3.34E-54
2	PRF1	5.34E-43	2.7532237	None	None	1.05E-39
2	GNLY	2.09E-51	5.346766	None	None	8.19E-48
2	GZMB	6.94E-49	4.825422	None	None	1.94E-45
2	KLRB1	3.69E-65	4.6943645	None	None	3.61E-61
2	KLRD1	7.21E-65	4.658858	None	None	4.71E-61
2	CD7	1.39E-38	2.578242	None	None	2.48E-35
2	TYROBP	4.09E-71	4.788502	None	None	8.02E-67
2	IFITM1	4.49E-43	2.0724273	None	None	9.77E-40
3	RPS18	4.31E-31	0.9185822	None	None	8.86E-28
3	TPT1	4.83E-48	1.0312977	None	None	4.73E-44
3	RPS21	5.77E-30	0.8176789	None	None	1.03E-26
3	RPL34	4.53E-31	0.78957444	None	None	8.86E-28
3	EEF1A1	1.37E-31	0.7429849	None	None	3.35E-28
3	CD52	9.12E-33	1.6407712	None	None	2.55E-29
3	RPS12	1.02E-34	0.83967346	None	None	3.33E-31
3	RPL11	7.64E-30	0.7732554	None	None	1.25E-26
3	IL7R	5.32E-64	4.3823886	None	None	1.04E-59
3	RPL39	7.99E-35	0.8586577	None	None	3.13E-31
4	CCL5	1.50E-10	1.5324768	None	None	1.83E-07
4	RPS15A	5.71E-16	0.70234215	None	None	1.40E-12
4	RPL30	9.56E-11	0.6117119	None	None	1.25E-07
4	GZMA	2.26E-11	1.3514099	None	None	3.16E-08
4	RPL13	1.19E-11	0.62245244	None	None	1.80E-08
4	GZMK	1.83E-10	1.7762877	None	None	2.10E-07
4	RPS29	2.14E-15	0.71739125	None	None	4.65E-12
4	RPS27	4.82E-10	0.6376603	None	None	5.13E-07
4	CD8A	1.16E-14	1.8838103	None	None	2.06E-11
4	ITM2C	2.55E-13	1.8598926	None	None	4.16E-10
5	RPS27	1.64E-09	0.54968554	None	None	3.08E-06
5	GZMH	3.59E-17	2.4067864	None	None	3.51E-13
5	NKG7	1.93E-10	1.6874326	None	None	4.20E-07
5	GNLY	9.50E-16	3.5410347	None	None	6.20E-12
5	KLRD1	3.36E-20	2.954916	None	None	6.58E-16
5	LINC01871	1.98E-09	1.9404677	None	None	3.23E-06
5	GZMB	7.76E-11	2.317063	None	None	2.17E-07
5	LINC02446	1.46E-10	3.6470053	None	None	3.56E-07
5	RPS6	6.38E-11	0.6022945	None	None	2.08E-07
5	KLRC4	1.73E-09	2.0416493	None	None	3.08E-06
6	CTLA4	1.18E-21	4.052064	None	None	2.89E-18
6	IL32	3.45E-23	2.5125043	None	None	1.13E-19
6	CARD16	4.59E-14	2.2525911	None	None	5.28E-11
6	TIGIT	2.37E-21	3.266728	None	None	5.16E-18
6	STAM	2.69E-14	3.8652322	None	None	3.29E-11
6	LTB	1.62E-15	2.6679225	None	None	2.26E-12
6	FOXP3	7.32E-18	9.159914	None	None	1.19E-14
6	TBC1D4	1.51E-17	4.3926163	None	None	2.27E-14
6	CORO1B	3.15E-13	2.5346668	None	None	3.43E-10
6	S100A4	9.31E-22	2.1557431	None	None	2.60E-18
7	CST3	1.51E-22	6.2584434	None	None	1.10E-19
7	NAMPT	1.03E-23	4.1905336	None	None	1.01E-20
7	PSAP	4.10E-21	3.1675982	None	None	2.36E-18
7	CD83	3.10E-23	5.0118585	None	None	2.64E-20
7	SIPA1L1	2.28E-21	4.4120255	None	None	1.40E-18
7	HLA-DRB1	1.30E-22	3.0379484	None	None	9.78E-20
7	PLAUR	1.69E-24	7.2549734	None	None	2.07E-21
7	PLXDC2	8.99E-26	6.1526904	None	None	1.60E-22
7	IFI30	6.43E-26	6.7624307	None	None	1.40E-22
7	SAT1	1.14E-28	3.7164762	None	None	4.46E-25
8	MALAT1	2.14E-17	1.2483206	None	None	5.75E-15
8	RFTN1	4.39E-13	3.3341734	None	None	6.93E-11
8	ATXN1	8.97E-14	3.1262498	None	None	1.49E-11
8	SIK3	1.24E-15	3.2614639	None	None	2.58E-13
8	VPS13B	1.35E-12	3.4789383	None	None	2.02E-10
8	PRKCH	8.11E-16	2.588867	None	None	1.76E-13
8	CHST11	5.72E-16	3.4933808	None	None	1.30E-13
8	PPP1R16B	1.64E-16	3.455277	None	None	3.91E-14
8	RABGAP1L	9.28E-13	2.690405	None	None	1.41E-10
8	ANKRD11	3.10E-12	3.3661482	None	None	4.44E-10
9	HSP90B1	1.30E-18	4.0922146	None	None	4.23E-15
9	DERL3	5.57E-16	8.958008	None	None	5.45E-13
9	HERPUD1	1.71E-16	3.479165	None	None	2.39E-13
9	JCHAIN	2.57E-18	11.681846	None	None	7.19E-15
9	IGHG1	1.84E-19	9.02507	None	None	9.00E-16
9	IGLC2	7.61E-21	8.940172	None	None	4.97E-17
9	IGHV4-61	7.57E-21	11.205306	None	None	4.97E-17
9	IGLV3-1	7.57E-21	11.531429	None	None	4.97E-17
9	MZB1	7.13E-15	8.971891	None	None	3.58E-12
9	IGHGP	1.24E-13	7.670989	None	None	4.33E-11
0	IL7R	2.51E-145	2.2112777	None	None	4.93E-141
0	ARHGAP15	4.77E-93	1.21048	None	None	2.35E-89
0	GSTK1	1.87E-81	1.2605145	None	None	6.13E-78
0	RPLP0	3.16E-77	0.6485295	None	None	8.88E-74
0	FXYD5	8.89E-102	0.88010514	None	None	5.83E-98
0	LDHB	2.12E-73	1.2445947	None	None	5.21E-70
0	RPL28	5.20E-70	0.3845244	None	None	1.14E-66
0	VIM	2.38E-136	1.2783217	None	None	2.34E-132
0	EEF1A1	2.25E-68	0.42316678	None	None	4.43E-65
0	TPT1	5.38E-89	0.55726147	None	None	2.12E-85
1	FOS	8.50E-143	3.299096	None	None	2.78E-139
1	KLF6	1.12E-155	2.204935	None	None	4.40E-152
1	BTG1	3.69E-92	1.0947901	None	None	7.26E-89
1	PPP1R15A	3.56E-122	2.4043598	None	None	8.75E-119
1	GPR183	4.78E-125	2.4232025	None	None	1.34E-121
1	JUN	7.95E-207	3.3718753	None	None	1.56E-202
1	DNAJB1	2.44E-187	2.7153752	None	None	2.40E-183
1	DUSP1	2.99E-168	2.9106822	None	None	1.96E-164
1	TUBA1A	3.40E-101	2.0591981	None	None	7.44E-98
1	CD69	2.42E-164	2.3295143	None	None	1.19E-160
2	RPL32	2.07E-97	0.62069005	None	None	8.13E-94
2	RPS14	5.19E-90	0.55725265	None	None	1.28E-86
2	RPL34	3.33E-94	0.5230986	None	None	9.36E-91
2	RPS15A	1.74E-99	0.54048795	None	None	8.55E-96
2	RPS12	8.20E-101	0.6837594	None	None	5.38E-97
2	RPL30	2.40E-116	0.58414495	None	None	4.71E-112
2	RPS27A	5.37E-86	0.4821179	None	None	1.06E-82
2	RPS3A	3.97E-115	0.6580665	None	None	3.91E-111
2	RPS13	4.84E-97	0.6166077	None	None	1.58E-93
2	RPS3	2.06E-85	0.5812438	None	None	3.68E-82
3	RPS28	2.03E-77	0.57107174	None	None	5.71E-74
3	RPS3A	7.20E-88	0.6483365	None	None	1.42E-83
3	RPL30	1.65E-82	0.5781324	None	None	6.50E-79
3	RPS3	1.36E-75	0.61251014	None	None	3.34E-72
3	RPS14	2.14E-81	0.586418	None	None	7.02E-78
3	RPS15A	4.14E-83	0.5487287	None	None	2.04E-79
3	RPS12	1.95E-69	0.6559366	None	None	3.84E-66
3	RPS13	4.36E-70	0.59637195	None	None	9.52E-67
3	MT-CYB	1.48E-67	0.5387384	None	None	2.65E-64
3	RPL32	1.42E-84	0.64040345	None	None	1.40E-80
4	HLA-DQA1	5.71E-196	4.989005	None	None	1.60E-192
4	CD37	2.76E-201	2.3092122	None	None	1.09E-197
4	HLA-DRA	2.15E-201	5.857147	None	None	1.06E-197
4	AFF3	8.38E-182	5.631785	None	None	1.83E-178
4	CD74	5.61E-208	5.249994	None	None	1.10E-203
4	HLA-DPA1	7.98E-180	4.22183	None	None	1.57E-176
4	HLA-DRB1	2.35E-203	5.040215	None	None	2.31E-199
4	CD83	2.14E-202	6.011839	None	None	1.40E-198
4	HLA-DQB1	5.94E-193	5.0793343	None	None	1.46E-189
4	CD79A	2.58E-199	6.066798	None	None	8.45E-196
5	PFN1	3.98E-139	1.6963154	None	None	7.11E-136
5	CD247	4.45E-164	3.378668	None	None	1.75E-160
5	HLA-C	1.15E-136	1.455492	None	None	1.50E-133
5	PRF1	2.34E-168	5.595012	None	None	1.15E-164
5	GNLY	8.11E-156	6.907666	None	None	2.28E-152
5	NKG7	2.39E-174	6.789874	None	None	4.66E-170
5	CCL5	2.41E-139	5.5919375	None	None	4.73E-136
5	CST7	4.74E-174	5.3132477	None	None	4.66E-170
5	GZMA	2.06E-156	5.1676054	None	None	6.76E-153
5	GZMB	2.19E-172	5.8165455	None	None	1.43E-168
6	HLA-B	7.31E-115	1.1185942	None	None	2.40E-111
6	NKG7	4.63E-119	6.0674906	None	None	1.82E-115
6	S100A4	1.34E-109	2.6578305	None	None	3.70E-106
6	SH3BGRL3	3.58E-106	1.808227	None	None	7.82E-103
6	CST7	1.51E-109	4.1388907	None	None	3.70E-106
6	CD99	1.74E-103	1.9738711	None	None	3.42E-100
6	CCL5	2.87E-124	5.5240245	None	None	1.41E-120
6	GZMH	2.50E-149	5.6042433	None	None	4.92E-145
6	B2M	7.26E-138	1.1427706	None	None	4.76E-134
6	HLA-C	8.78E-140	1.6458231	None	None	8.63E-136
7	IFI30	1.17E-147	7.874691	None	None	3.84E-144
7	NEAT1	4.91E-144	4.2463756	None	None	1.02E-140
7	CST3	7.97E-148	8.222568	None	None	3.13E-144
7	TYROBP	1.25E-142	6.1816654	None	None	2.23E-139
7	S100A9	9.86E-150	9.510178	None	None	4.85E-146
7	FCN1	2.19E-145	8.499745	None	None	6.16E-142
7	CTSS	4.83E-145	4.7075863	None	None	1.19E-141
7	FTL	1.19E-152	2.8901975	None	None	2.35E-148
7	LYZ	4.18E-151	9.573528	None	None	4.11E-147
7	S100A8	7.41E-151	10.113356	None	None	4.86E-147
8	HLA-DPB1	7.89E-72	3.566425	None	None	1.72E-68
8	CD74	5.97E-83	4.7717204	None	None	3.92E-79
8	HLA-DQB1	4.20E-72	3.9663408	None	None	1.03E-68
8	HLA-DPA1	2.84E-69	3.5505881	None	None	5.58E-66
8	HLA-DQA1	1.67E-79	4.1541796	None	None	6.56E-76
8	HLA-DRA	1.52E-80	5.079043	None	None	7.46E-77
8	BLK	8.48E-74	5.0119877	None	None	2.38E-70
8	MS4A1	2.43E-87	5.098299	None	None	4.77E-83
8	BANK1	5.33E-86	4.976401	None	None	5.24E-82
8	CD79A	7.58E-76	4.572542	None	None	2.48E-72
9	CD8B	2.53E-53	5.2773147	None	None	1.66E-49
9	CD8A	5.36E-57	5.2543373	None	None	5.27E-53
9	CMC1	1.12E-45	3.983456	None	None	3.15E-42
9	HLA-B	5.47E-45	0.98704475	None	None	1.34E-41
9	LYAR	1.80E-43	3.7170336	None	None	3.54E-40
9	CCL5	6.25E-59	5.468395	None	None	1.23E-54
9	CST7	4.23E-52	4.1765018	None	None	2.08E-48
9	NKG7	1.15E-49	5.4816914	None	None	4.53E-46
9	GZMA	1.57E-44	3.7907286	None	None	3.43E-41
9	HCST	1.77E-46	2.2789	None	None	5.79E-43
10	STAM	1.50E-12	3.3292265	None	None	1.41E-09
10	PFN1	1.40E-13	0.7889027	None	None	2.30E-10
10	TMSB10	4.77E-14	0.568754	None	None	1.34E-10
10	TMSB4X	3.82E-13	0.42634663	None	None	4.70E-10
10	IL32	5.40E-23	1.9241086	None	None	1.06E-18
10	ARHGDIB	1.56E-12	0.587481	None	None	1.41E-09
10	FOXP3	3.58E-12	8.860203	None	None	2.62E-09
10	S100A10	8.13E-14	1.3168097	None	None	1.78E-10
10	HLA-A	1.61E-12	0.62596434	None	None	1.41E-09
10	ANXA2	1.65E-12	1.9809965	None	None	1.41E-09
11	CD8B	7.58E-09	3.3541262	None	None	0.000149075
11	RPL26	3.91E-08	0.29138806	None	None	0.000384392
11	RPS14	1.12E-05	0.32948142	None	None	0.024510731
11	KLRC4	2.62E-05	2.740191	None	None	0.051530939
11	TRBV24-1	1.09E-06	5.8341036	None	None	0.004287207
11	RPL24	3.04E-05	0.32054847	None	None	0.054363339
11	IL32	5.60E-06	1.1862634	None	None	0.015726962
11	CD8A	8.78E-07	2.490481	None	None	0.004287207
11	RPS3	4.24E-07	0.42199552	None	None	0.002780016
11	RPS12	3.69E-06	0.4048951	None	None	0.012076494
12	DERL3	1.65E-18	8.256556	None	None	1.26E-15
12	UBE2J1	1.49E-16	3.9860604	None	None	4.96E-14
12	HSP90B1	6.65E-18	3.5495574	None	None	3.85E-15
12	MZB1	7.65E-20	8.382848	None	None	3.56E-16
12	CD27	9.77E-17	3.8403926	None	None	3.62E-14
12	PPIB	6.62E-17	2.761966	None	None	2.66E-14
12	JCHAIN	3.45E-21	10.34949	None	None	3.63E-17
12	CD79A	3.25E-19	5.6546	None	None	5.11E-16
12	ITM2C	3.05E-19	6.062669	None	None	5.11E-16
12	SSR4	3.69E-21	3.5578518	None	None	3.63E-17
13	IL2RB	3.67E-11	4.8410497	None	None	1.20E-07
13	XCL1	1.56E-15	7.1136217	None	None	3.07E-11
13	IFITM2	4.29E-12	2.2455692	None	None	1.69E-08
13	IFITM3	3.54E-10	3.4651403	None	None	6.96E-07
13	CMC1	1.28E-10	4.169739	None	None	3.14E-07
13	H3F3B	2.74E-10	1.3768039	None	None	5.98E-07
13	GNLY	3.52E-13	6.73575	None	None	2.31E-09
13	HOPX	2.24E-13	4.472571	None	None	2.21E-09
13	GZMK	7.98E-13	6.675137	None	None	3.92E-09
13	KLRC1	7.35E-11	6.961793	None	None	2.07E-07
14	CD8A	2.78E-10	4.9565263	None	None	1.09E-06
14	CD8B	5.56E-09	4.5659113	None	None	1.11E-05
14	NKG7	5.65E-09	5.6439366	None	None	1.11E-05
14	TRBV7-5	2.89E-13	9.324483	None	None	5.68E-09
14	HLA-C	3.06E-09	1.4422654	None	None	8.35E-06
14	TRBV7-6	1.45E-10	7.0643992	None	None	7.11E-07
14	TRAV3	8.56E-12	7.5732427	None	None	8.42E-08
14	B2M	3.40E-09	0.99904764	None	None	8.35E-06
14	KLRD1	1.44E-09	3.8818612	None	None	4.71E-06
14	GZMH	8.48E-11	4.9700327	None	None	5.56E-07
15	MS4A7	5.80E-12	7.757548	None	None	1.78E-08
15	FCER1G	7.46E-12	6.388306	None	None	1.78E-08
15	AIF1	5.47E-12	6.2480545	None	None	1.78E-08
15	CD68	9.08E-12	6.646105	None	None	1.78E-08
15	FTH1	8.11E-12	3.2668579	None	None	1.78E-08
15	LST1	4.76E-12	7.174252	None	None	1.78E-08
15	SERPINA1	6.90E-12	7.3156815	None	None	1.78E-08
15	FCGR3A	9.11E-12	6.3301167	None	None	1.78E-08
15	PSAP	7.22E-12	4.798867	None	None	1.78E-08
15	IFITM3	6.68E-12	6.681832	None	None	1.78E-08
16	APP	1.04E-08	5.6864324	None	None	2.54E-05
16	SPIB	1.38E-08	6.3939476	None	None	2.54E-05
16	IRF7	1.08E-08	6.2656045	None	None	2.54E-05
16	ITM2C	1.05E-08	7.197411	None	None	2.54E-05
16	PLD4	9.98E-09	9.051607	None	None	2.54E-05
16	CLEC4C	9.79E-09	12.382282	None	None	2.54E-05
16	LILRA4	9.70E-09	11.631087	None	None	2.54E-05
16	SERPINF1	9.70E-09	11.158938	None	None	2.54E-05
16	SEC61B	1.42E-08	3.550174	None	None	2.54E-05
16	IGKC	1.53E-08	7.029117	None	None	2.54E-05
0	RPS3A	5.62E-89	0.87645334	None	None	1.06E-84
0	RPS13	8.66E-78	0.7738425	None	None	4.10E-74
0	RPL30	9.83E-88	0.857443	None	None	9.30E-84
0	RPL11	7.31E-75	0.80032	None	None	2.77E-71
0	RPS23	1.83E-66	0.784089	None	None	3.47E-63
0	RPL32	1.61E-72	0.84357446	None	None	4.35E-69
0	RPL34	6.62E-74	0.75540817	None	None	2.09E-70
0	RPS15A	5.63E-72	0.7603704	None	None	1.33E-68
0	RPS12	7.49E-67	0.97172475	None	None	1.58E-63
0	RPS14	1.45E-80	0.804537	None	None	9.12E-77
1	CST7	7.87E-118	6.0822873	None	None	2.48E-114
1	GZMB	9.00E-125	8.009166	None	None	5.68E-121
1	CD7	1.45E-120	4.1575823	None	None	5.49E-117
1	KLRB1	8.30E-106	5.0793986	None	None	1.96E-102
1	CTSW	1.45E-123	5.6198587	None	None	6.87E-120
1	HLA-C	1.81E-102	1.3509313	None	None	3.11E-99
1	FGFBP2	4.16E-108	7.2036853	None	None	1.12E-104
1	CD247	5.54E-101	3.5199487	None	None	8.73E-98
1	GNLY	7.02E-129	8.155408	None	None	6.64E-125
1	NKG7	4.09E-133	7.947705	None	None	7.74E-129
2	RPS29	1.09E-34	0.7827201	None	None	2.29E-31
2	RPLP0	2.08E-34	0.7685144	None	None	3.65E-31
2	LDHB	3.62E-35	1.5481172	None	None	1.14E-31
2	TPT1	6.18E-35	0.5280897	None	None	1.58E-31
2	RPL36	4.24E-40	0.7833499	None	None	2.00E-36
2	IL32	5.05E-41	2.2458375	None	None	3.18E-37
2	RPS18	3.26E-45	0.8764736	None	None	3.09E-41
2	RPL23A	2.77E-38	0.71997595	None	None	1.05E-34
2	LTB	6.68E-35	1.89922	None	None	1.58E-31
2	IL7R	1.78E-49	2.5185404	None	None	3.37E-45
3	S100A9	1.47E-115	8.743644	None	None	2.78E-111
3	VCAN	1.84E-108	7.5036645	None	None	6.96E-105
3	FTL	1.09E-102	2.86229	None	None	1.73E-99
3	CTSS	2.91E-107	4.6273723	None	None	7.88E-104
3	S100A8	3.90E-113	8.823406	None	None	2.46E-109
3	LYZ	1.61E-114	8.349073	None	None	1.52E-110
3	S100A12	4.63E-103	9.060777	None	None	7.97E-100
3	TKT	8.73E-107	3.9082205	None	None	2.06E-103
3	FCN1	1.34E-107	6.7497897	None	None	4.22E-104
3	S100A6	7.87E-106	3.6314807	None	None	1.65E-102
4	CD3E	1.09E-15	1.6274244	None	None	4.13E-12
4	RPLP0	2.17E-10	0.5282973	None	None	3.16E-07
4	B2M	3.33E-15	0.52997816	None	None	9.01E-12
4	IL32	1.18E-21	2.0893369	None	None	2.23E-17
4	RORA	1.50E-15	1.7493812	None	None	4.73E-12
4	CD52	1.70E-17	1.152103	None	None	1.19E-13
4	CCL5	1.03E-15	2.767998	None	None	4.13E-12
4	CD3D	1.54E-11	1.4759442	None	None	2.65E-08
4	RPS19	1.89E-17	0.5699146	None	None	1.19E-13
4	LYAR	2.22E-12	2.2703052	None	None	5.26E-09
5	CD37	1.65E-57	2.08052	None	None	5.22E-54
5	BANK1	2.18E-49	7.153289	None	None	4.12E-46
5	MS4A1	4.30E-70	8.915348	None	None	8.14E-66
5	HLA-DPB1	9.20E-50	3.664102	None	None	1.93E-46
5	CD79A	1.12E-67	7.807917	None	None	1.06E-63
5	CD74	4.14E-63	4.2262626	None	None	2.61E-59
5	EZR	4.60E-53	3.2247462	None	None	1.24E-49
5	HLA-DRA	2.20E-51	4.5549164	None	None	5.21E-48
5	LAPTM5	1.53E-57	1.976506	None	None	5.22E-54
5	HLA-DQB1	2.03E-58	4.1468525	None	None	9.62E-55
6	RPL32	3.54E-19	0.7124596	None	None	8.37E-16
6	RPL30	2.37E-25	0.76759595	None	None	4.47E-21
6	RPS3	3.65E-20	0.79766	None	None	9.88E-17
6	RPS15A	2.80E-20	0.6718685	None	None	8.84E-17
6	RPS12	4.41E-19	0.8394096	None	None	8.57E-16
6	RPS28	5.65E-25	0.6846366	None	None	5.34E-21
6	RPL11	1.16E-20	0.69621104	None	None	4.41E-17
6	RPS3A	1.30E-21	0.72159636	None	None	8.23E-18
6	RPL19	3.85E-21	0.66388214	None	None	1.82E-17
6	RPL35A	4.53E-19	0.62362576	None	None	8.57E-16
7	AIF1	1.20E-32	4.765202	None	None	1.14E-28
7	HLA-DRA	1.96E-30	5.0203176	None	None	4.13E-27
7	COTL1	2.61E-32	3.556084	None	None	1.64E-28
7	LST1	1.12E-30	4.790188	None	None	2.65E-27
7	HLA-DRB1	2.46E-30	4.7843356	None	None	4.66E-27
7	CST3	8.09E-35	5.9356055	None	None	1.53E-30
7	HLA-DPA1	7.65E-32	4.633556	None	None	2.07E-28
7	FTL	4.25E-32	2.8219476	None	None	2.01E-28
7	IFI30	6.70E-32	5.3889246	None	None	2.07E-28
7	NPC2	5.56E-32	3.3884468	None	None	2.07E-28
8	IL32	6.45E-19	3.2280726	None	None	1.22E-14
8	TMSB10	5.74E-09	0.7033607	None	None	1.55E-05
8	CD3D	2.25E-08	1.9716636	None	None	4.73E-05
8	CD3E	3.29E-09	1.8751713	None	None	1.04E-05
8	CD52	1.59E-16	1.853472	None	None	1.50E-12
8	HLA-A	3.03E-11	1.0226663	None	None	1.43E-07
8	ARID5B	1.73E-09	2.4899092	None	None	6.54E-06
8	S100A10	3.19E-08	1.4241984	None	None	6.04E-05
8	B2M	3.86E-13	0.81269616	None	None	2.43E-09
8	PTPRCAP	1.38E-08	1.4979641	None	None	3.26E-05
9	SSR2	0.002731384	0.97261494	None	None	1
9	SMARCA5	0.000158123	1.9254454	None	None	1
9	PMAIP1	0.001891249	1.5407941	None	None	1
9	XBP1	0.000299077	1.6293418	None	None	1
9	SSR3	0.002846649	1.438412	None	None	1
9	RPLP0	0.001175886	0.6154208	None	None	1
9	GATA3	0.000588037	2.268307	None	None	1
9	ISG20	0.002199711	1.3481435	None	None	1
9	FKBP11	6.16E-05	1.966229	None	None	1
9	PBX4	0.00242081	1.6786324	None	None	1
10	IGKV4-1	2.62E-09	10.275751	None	None	4.01E-05
10	FCER2	8.04E-07	6.274424	None	None	0.001521165
10	TCF4	9.05E-08	5.465468	None	None	0.000285503
10	CD37	8.79E-09	2.4452133	None	None	5.54E-05
10	CD74	3.31E-08	4.0078645	None	None	0.000125354
10	CD79A	4.24E-09	5.3307395	None	None	4.01E-05
10	IRF8	2.06E-08	3.8722181	None	None	9.75E-05
10	FCMR	1.27E-07	3.6891859	None	None	0.000344304
10	LAPTM5	4.06E-07	1.6235116	None	None	0.000852526
10	ADAM28	1.76E-07	4.930439	None	None	0.000417269
11	RPL9	5.86E-07	0.96369505	None	None	0.002219325
11	RPL32	2.17E-06	0.8850769	None	None	0.004567422
11	RPL22	1.67E-06	0.9102057	None	None	0.00421081
11	RPS6	1.05E-06	1.0396904	None	None	0.003314688
11	RPS3A	4.61E-07	0.94154793	None	None	0.00217967
11	RPL3	3.40E-07	1.1388178	None	None	0.002143557
11	CD8B	4.12E-09	7.00114	None	None	3.89E-05
11	LINC02446	3.17E-09	8.152682	None	None	3.89E-05
11	RPS9	3.25E-06	0.8908142	None	None	0.006143139
11	RPS12	4.15E-06	0.9840155	None	None	0.007136051
0	KLRD1	1.36E-52	4.364563	None	None	2.31E-48
0	KLRF1	2.65E-45	4.423493	None	None	4.50E-42
0	PRF1	7.89E-46	4.420697	None	None	1.67E-42
0	CD7	3.71E-46	2.7904139	None	None	9.00E-43
0	HOPX	3.20E-46	4.1623616	None	None	9.00E-43
0	NKG7	5.56E-47	5.1364713	None	None	1.88E-43
0	SPON2	5.97E-48	4.7731037	None	None	2.53E-44
0	GZMB	2.97E-49	4.7213893	None	None	1.68E-45
0	GNLY	5.99E-51	5.707302	None	None	5.08E-47
0	ZFP36	3.73E-45	2.760976	None	None	5.53E-42
1	NAMPT	2.28E-61	6.5459337	None	None	3.01E-58
1	IFI30	3.72E-65	8.6401205	None	None	3.16E-61
1	LYZ	1.16E-62	9.03827	None	None	3.28E-59
1	S100A9	7.95E-66	8.915977	None	None	1.35E-61
1	AIF1	3.18E-62	6.302813	None	None	7.72E-59
1	LGALS3	1.80E-61	6.3386064	None	None	3.01E-58
1	SAT1	1.02E-63	4.327248	None	None	4.34E-60
1	S100A8	2.19E-61	9.837839	None	None	3.01E-58
1	CST3	6.43E-64	8.814244	None	None	3.64E-60
1	FTH1	1.01E-61	3.0582314	None	None	2.15E-58
2	RPS18	1.51E-17	0.6954782	None	None	2.32E-14
2	RPL32	6.43E-19	0.51714724	None	None	1.36E-15
2	LEF1	7.75E-17	2.1566038	None	None	9.39E-14
2	APOO	4.26E-18	0.9316046	None	None	7.22E-15
2	RPS3A	2.07E-17	0.5300557	None	None	2.93E-14
2	NDFIP1	7.82E-21	1.8346488	None	None	3.32E-17
2	RPS25	1.65E-19	0.6549677	None	None	5.61E-16
2	SARAF	8.64E-17	1.027354	None	None	9.71E-14
2	IL7R	4.22E-24	2.4419491	None	None	3.58E-20
2	RPS8	2.54E-17	0.5967577	None	None	3.31E-14
3	IL32	5.39E-14	1.8199782	None	None	1.31E-10
3	IL7R	7.62E-15	1.9729218	None	None	6.46E-11
3	RPS19	1.68E-12	0.6983155	None	None	3.16E-09
3	RPL18A	1.23E-13	0.6639751	None	None	2.60E-10
3	TPT1	4.35E-14	0.6650598	None	None	1.23E-10
3	RPL36A	2.17E-14	0.78471667	None	None	7.38E-11
3	RPS18	1.82E-14	0.87945944	None	None	7.38E-11
3	RPL13A	2.31E-12	0.74741006	None	None	3.92E-09
3	LTB	2.76E-15	1.9631588	None	None	4.67E-11
3	LDHB	1.58E-14	1.3791918	None	None	7.38E-11
4	RPS5	8.97E-20	1.0001582	None	None	1.69E-16
4	RPL34	1.43E-23	0.6844031	None	None	2.42E-19
4	EEF1A1	4.39E-20	0.7018286	None	None	9.31E-17
4	RPS6	4.13E-20	0.90178823	None	None	9.31E-17
4	RPS3A	6.04E-21	0.7379436	None	None	1.71E-17
4	RPS12	1.26E-19	0.8399196	None	None	2.14E-16
4	RPL11	2.70E-22	0.7292095	None	None	1.52E-18
4	RPL30	1.62E-19	0.65112835	None	None	2.49E-16
4	TPT1	4.13E-22	0.7992853	None	None	1.75E-18
4	RPL13	1.14E-21	0.8753029	None	None	3.88E-18
5	RPS15A	6.59E-20	0.65838724	None	None	1.40E-16
5	RPL32	2.33E-23	0.80885607	None	None	1.98E-19
5	RPL22	1.41E-20	0.7967931	None	None	3.42E-17
5	RPL11	4.68E-23	0.73111933	None	None	2.64E-19
5	RPL30	9.09E-24	0.6592206	None	None	1.54E-19
5	RPL34	5.43E-19	0.63680226	None	None	9.20E-16
5	RPL19	1.08E-21	0.65235656	None	None	3.67E-18
5	RPS3A	3.09E-21	0.69658417	None	None	8.74E-18
5	RPL39	2.52E-22	0.73921293	None	None	1.07E-18
5	RPS13	2.75E-19	0.68425626	None	None	5.18E-16
6	B2M	3.71E-36	0.8834058	None	None	2.10E-32
6	GZMH	2.03E-45	5.114775	None	None	3.45E-41
6	GZMA	4.09E-29	3.3462749	None	None	7.71E-26
6	NKG7	1.15E-33	5.0129156	None	None	4.87E-30
6	HLA-B	3.22E-29	0.9358959	None	None	6.82E-26
6	CTSW	1.58E-30	3.0270524	None	None	3.83E-27
6	FGFBP2	1.77E-32	4.13854	None	None	6.00E-29
6	CST7	1.03E-30	3.6147432	None	None	2.91E-27
6	GNLY	7.41E-28	4.6362963	None	None	1.26E-24
6	CCL5	1.77E-39	4.757439	None	None	1.50E-35
7	CXCR4	1.66E-10	1.5376891	None	None	3.52E-07
7	CD8B	3.73E-13	3.8231347	None	None	1.27E-09
7	PIK3R1	4.31E-10	1.5859394	None	None	7.32E-07
7	LYAR	6.11E-11	2.4015737	None	None	1.73E-07
7	HERPUD1	3.19E-10	1.9766296	None	None	6.01E-07
7	CD74	1.24E-10	1.6062396	None	None	3.00E-07
7	CCL5	1.13E-14	3.4674582	None	None	4.77E-11
7	GZMK	8.67E-17	4.9380703	None	None	4.90E-13
7	DUSP2	2.62E-18	3.0250275	None	None	2.22E-14
7	CD8A	2.60E-18	3.7418184	None	None	2.22E-14
8	RPL4	0.000624818	1.1129669	None	None	1
8	P2RY8	0.003069797	1.9722375	None	None	1
8	TPT1	0.003032494	0.82129604	None	None	1
8	RAB6A	0.002944646	2.163459	None	None	1
8	IKBKE	0.003735364	2.8341668	None	None	1
8	EEF1G	0.002887378	1.030585	None	None	1
8	AC079793.1	0.002655157	3.136527	None	None	1
8	HINT1	0.001601522	1.2146907	None	None	1
8	SMIM20	0.003441459	2.9384484	None	None	1
8	RPS18	0.003826049	1.0241289	None	None	1
9	HLA-DRA	8.75E-06	6.1749983	None	None	0.02749053
9	HLA-DPB1	1.30E-05	4.6975446	None	None	0.02749053
9	HLA-DQB1	1.16E-05	5.152171	None	None	0.02749053
9	HLA-DPA1	1.06E-05	4.8336716	None	None	0.02749053
9	ARHGAP24	8.62E-06	6.2782955	None	None	0.02749053
9	CD79A	5.48E-06	9.925275	None	None	0.02749053
9	NIBAN3	5.11E-06	31.677961	None	None	0.02749053
9	CD74	5.11E-06	5.221729	None	None	0.02749053
9	CD37	2.42E-05	2.3476336	None	None	0.045564768
9	CYB561A3	2.69E-05	4.2174163	None	None	0.045630368

SkewC Quality Control Output Plots

Select Run