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3k Pan T Cells from a Healthy Donor

Pan T cells isolated from mononuclear cells of a healthy donor (same donor as t_4k). T cells are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

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Accession Estimated No. of Cells Mean Reads per Cell Median Genes per Cell No. of Reads % Valid Barcodes % sequencing Saturation % Q30 Bases in Barcode % Q30 Bases in UMI % Reads Mapped to Genome % Reads Mapped Confidently to Genome % Reads Mapped Confidently to Intergenic Regions % Reads Mapped Confidently to Intronic Regions % Reads Mapped Confidently to Exonic Regions % Reads Mapped Confidently to Transcriptome % Reads Mapped Antisense to Gene % Fraction Reads in Cells Total Genes Detected Median UMI Counts Per Cell
3742 96520 1061 361179337 98.4 92.5 97.6 97.7 94.8 92.6 3.1 22.5 67.1 63.1 1.1 89.0 18586 3596

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Top 10 Marker Genes per Cluster

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Accession Seurat Cluster Gene Symbol P Value Average Log Pct 1 Pct 2 Adjusted P Value
0 YPEL5 1.27181948853062e-107 0.879515426159954 0.976 0.696 4.26542820063399e-103
0 GPR183 7.41189473277967e-67 0.921101653933235 0.668 0.346 2.48580125547965e-62
0 CCL5 3.37623084728089e-63 -2.89184489212017 0.063 0.357 1.13232030156106e-58
0 MYO1F 1.3688848231298e-62 -1.34816237925915 0.025 0.314 4.59096591981271e-58
0 ZNF331 2.13645605464533e-57 0.725135599941187 0.769 0.469 7.16524631606951e-53
0 CCR7 5.04793195471717e-57 0.684660980732103 0.61 0.309 1.69297541897304e-52
0 CD8B 1.08490032241672e-56 -1.38797104523774 0.025 0.293 3.6385387013212e-52
0 KLRB1 2.35295209726583e-54 -2.35691106949243 0.051 0.317 7.89133074381015e-50
0 TSPYL1 3.22889243923366e-48 0.719232755211972 0.536 0.28 1.08290594627019e-43
0 LEPROTL1 3.70366533370771e-45 0.582297243384035 0.798 0.544 1.24213527961889e-40
1 LMNA 3.7248348700674e-205 2.05375970589095 0.742 0.201 1.24923511872321e-200
1 ANXA1 6.66279118153667e-120 1.14250505435531 0.914 0.602 2.23456690646377e-115
1 NFKBIA 4.43992096280289e-97 0.937376465684656 0.944 0.631 1.48906069250483e-92
1 AC020916.1 4.16438341768188e-93 0.919700952448768 0.63 0.244 1.39665091062215e-88
1 CRIP1 3.76594052010363e-87 0.886585802601937 0.938 0.669 1.26302113163236e-82
1 TNFAIP3 1.61907903665525e-84 0.888927004217374 0.917 0.635 5.43006727313437e-80
1 SRGN 2.73838114690683e-78 0.757412532377105 0.922 0.623 9.18398269049612e-74
1 PPP1R15A 2.78292593942682e-76 0.75601511455196 0.921 0.641 9.33337701564967e-72
1 S100A4 2.12654662093743e-75 0.690892412034123 0.957 0.675 7.13201205729996e-71
1 RGCC 7.14599477537801e-74 0.94440481190134 0.885 0.601 2.39662372776628e-69
2 LINC02446 0 2.18449305329615 0.74 0.065 0
2 CD8B 1.54178562303678e-253 1.65462870077957 0.854 0.142 5.17084062254076e-249
2 CD8A 5.94285949927135e-117 0.940954683570681 0.583 0.127 1.99311621886563e-112
2 YBX3 3.48184159844386e-100 0.850634347520179 0.39 0.066 1.1677400352861e-95
2 AIF1 4.1150023098048e-84 1.00547874209148 0.591 0.189 1.38008947466233e-79
2 YPEL5 2.05842566995503e-62 0.760084489202769 0.982 0.722 6.90354801189516e-58
2 NELL2 7.37154704309459e-55 0.689595646968496 0.435 0.138 2.47226944731306e-50
2 ZNF331 4.84754688984239e-47 0.721549840634695 0.835 0.488 1.62577027591534e-42
2 CD7 1.87553863056222e-34 0.572311785567381 0.811 0.53 6.29018145917956e-30
2 CD55 3.11594550506226e-33 0.541732271623231 0.555 0.264 1.04502580348778e-28
3 NKG7 0 4.14770351746154 0.921 0.071 0
3 GNLY 0 4.05052395243569 0.733 0.079 0
3 CCL5 0 3.54206884927547 0.969 0.195 0
3 CST7 0 2.44560932821221 0.706 0.046 0
3 GZMA 3.47430622904015e-290 2.31330764862018 0.75 0.095 1.16521282309549e-285
3 GZMH 3.79562043299848e-289 2.85119648021794 0.452 0.01 1.27297518081903e-284
3 KLRD1 1.98377780824568e-235 1.64080006273882 0.358 0.006 6.65319401329436e-231
3 FGFBP2 3.05020562802395e-220 2.07707671364447 0.348 0.008 1.02297796352667e-215
3 CCL4 5.90519198803129e-217 3.47818574763684 0.431 0.026 1.98048328894593e-212
3 GZMB 4.46997252233198e-187 1.73226411429691 0.271 0.002 1.4991393845397e-182
4 YPEL5 4.30506009759392e-64 -1.45907131277191 0.485 0.789 1.44383105553105e-59
4 ZNF331 1.91228117333287e-48 -1.42668017395959 0.192 0.575 6.41340859912379e-44
4 KLRB1 3.94935559226833e-41 0.92410083884679 0.537 0.227 1.32453487853495e-36
4 S100A11 1.87806878802244e-37 0.79746372927047 0.535 0.252 6.29866710126967e-33
4 NR4A2 1.35402850563774e-33 -1.05952581635367 0.301 0.618 4.54114080220786e-29
4 SELENOK 2.77365282862503e-23 -0.758091366516911 0.311 0.571 9.30227685664262e-19
5 SRGN 2.38286258870424e-76 -1.7250491275254 0.251 0.733 7.99164454999628e-72
5 ZFP36 2.55308144208738e-72 -1.71279607933043 0.434 0.822 8.56252454047265e-68
5 YPEL5 3.59650051775128e-69 -1.57408166784458 0.439 0.792 1.20619434364342e-64
5 NFKBIA 1.10232088394036e-64 -1.72200807133993 0.328 0.737 3.69696378055918e-60
5 TNFAIP3 1.73624035618164e-63 -1.58491542075263 0.344 0.733 5.823002906562e-59
5 PPP1R15A 2.56429243790604e-59 -1.41470774424028 0.339 0.739 8.60012397824928e-55
5 NR4A2 5.53363975175127e-55 -1.39641205570803 0.188 0.628 1.85587209994234e-50
5 RGCC 8.75676067664475e-48 -1.55466949764583 0.376 0.691 2.93684239573311e-43
5 ANXA1 1.11508063093925e-47 -1.44555444260982 0.349 0.702 3.73975742004406e-43
5 S100A4 3.2449301105919e-46 -1.62597797774783 0.508 0.758 1.08828466049031e-41
6 RPL21 2.41995216124926e-155 -1.80553119769469 0.748 1 8.11603555839775e-151
6 RPS3 2.11661996522489e-151 -1.65040102747545 0.688 1 7.09872003937124e-147
6 RPL7 1.24509125165228e-150 -1.69368013336123 0.655 0.998 4.1757870397914e-146
6 RPS2 1.36045210642443e-148 -1.56275526451436 0.748 1 4.56268427452625e-144
6 RPL30 3.01239921168971e-148 -1.7650718251811 0.558 0.999 1.0102984476165e-143
6 RPS14 7.78187579635064e-148 -1.679377235787 0.712 1 2.60988550458008e-143
6 RPL3 3.82831729270598e-146 -1.53992656899347 0.748 0.999 1.28394105362773e-141
6 RPS3A 1.89940512280263e-144 -1.65013528103286 0.679 1 6.37022490085547e-140
6 RPS6 2.27477058979373e-143 -1.58502058362615 0.745 1 7.6291256040502e-139
6 RPL9 1.14983553329251e-141 -1.71365166302232 0.579 0.999 3.85631841155641e-137
7 HLA-DRA 7.32524211904134e-170 3.90100573922743 0.593 0.025 2.45673970188408e-165
7 HLA-DRB1 3.48799122799168e-152 3.17011138612825 0.692 0.043 1.16980249804385e-147
7 HLA-DRB5 4.51171299871588e-109 1.12379264436685 0.275 0.007 1.51313830550933e-104
7 MIR4435-2HG 3.36167866416984e-94 0.918724376827447 0.407 0.022 1.12743979038928e-89
7 HLA-DQB1 5.51115620959471e-91 1.974376795965 0.352 0.017 1.84833156957388e-86
7 HLA-DQA2 1.5009525454167e-72 1.15209127755632 0.264 0.012 5.03389464681853e-68
7 CYTOR 1.78487422747816e-68 1.21490065942708 0.703 0.097 5.98611118411624e-64
7 MYL6B 2.12512032184783e-66 0.536533663129557 0.286 0.015 7.12722853541325e-62
7 PTMS 1.14185515044372e-65 0.495075828415882 0.275 0.014 3.82955380355816e-61
7 PARVB 1.33148010349012e-63 0.400625000608146 0.275 0.015 4.46551797108515e-59



SkewC Quality Control Output Plots


Gene Body Coverage

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Gene Body Coverage of All Cells.

Typical Coverage
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Gene Body Coverage of Typical Cells.
Skewed Coverage
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Gene Body Coverage of Skewed Cells.

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Cell Clustering Result.
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Mean Gene Body Coverage.