Single-cell ATAC-seq Control of Cross-contaminations.
On the Fluidigm C1 platform for single-cell analysis, the cells are captured in 96 chambers arranged serially, and then washed before further processing. Thus, debris present from the loading medium or released by captured cells upstream are a possible source of contamination. We generated a control datasets using the single-cell ATAC-seq protocol available from Fluidigm's ScriptHub. We cultivated human Hep G2 and mouse Hepa 1-6 (both are liver cancer cell lines), stained them with green and red calceins (respectively), and loaded them at equal concentration in a Fluidigm medium flow cell (old design), before running the C1 single-cell ATAC-seq program. To evaluate damage and carry-over of debris from FACS-sorting, two IFCs were run in two C1 machines in parallel. In the first (flow cell ID 1772-123-148) the cells were not FACSed, in the second (ID 1772-123-155), the cells were FACSed but not washed and in the third, they were FACSed and washed (ID 1772-123-158).