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PBMCs from BALB/c mice - 5’ gene expression

5’ gene expression data from Peripheral Blood Mononuclear Cells (PBMCs) pooled from BALB/c mice, purchased from CMLabs (catalog number: 12121912). Cells were ~77.5% viable by trypan blue stain. PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

CellRanger Metadata

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Estimated No. of Cells Mean Reads per Cell Median Genes per Cell No. of Reads % Valid Barcodes % sequencing Saturation % Q30 Bases in Barcode % Q30 Bases in UMI % Reads Mapped to Genome % Reads Mapped Confidently to Genome % Reads Mapped Confidently to Intergenic Regions % Reads Mapped Confidently to Intronic Regions % Reads Mapped Confidently to Exonic Regions % Reads Mapped Confidently to Transcriptome % Reads Mapped Antisense to Gene % Fraction Reads in Cells Total Genes Detected Median UMI Counts Per Cell
8000 70943 1235 567549380 90.0 85.2 95.9 95.5 90.1 87.5 8.4 7.5 74.0 67.7 5.1 96.7 18313 3683

Interactive UMAP Plot