PBMCs from BALB/c mice - 5’ gene expression
5’ gene expression data from Peripheral Blood Mononuclear Cells (PBMCs) pooled from BALB/c mice, purchased from CMLabs (catalog number: 12121912). Cells were ~77.5% viable by trypan blue stain. PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).
CellRanger Metadata
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| Estimated No. of Cells | Mean Reads per Cell | Median Genes per Cell | No. of Reads | % Valid Barcodes | % sequencing Saturation | % Q30 Bases in Barcode | % Q30 Bases in UMI | % Reads Mapped to Genome | % Reads Mapped Confidently to Genome | % Reads Mapped Confidently to Intergenic Regions | % Reads Mapped Confidently to Intronic Regions | % Reads Mapped Confidently to Exonic Regions | % Reads Mapped Confidently to Transcriptome | % Reads Mapped Antisense to Gene | % Fraction Reads in Cells | Total Genes Detected | Median UMI Counts Per Cell |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 8000 | 70943 | 1235 | 567549380 | 90.0 | 85.2 | 95.9 | 95.5 | 90.1 | 87.5 | 8.4 | 7.5 | 74.0 | 67.7 | 5.1 | 96.7 | 18313 | 3683 |