FastQCFastQC Report
Sun 19 Mar 2023
SRR4896114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205803
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG181328.810367195813471No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA22541.0952221299009246No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG18900.9183539598548127No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9700.47132451907892503No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC9080.4411986219831587No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG8710.423220264038911No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCA6780.32944126178918676No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4200.2040786577455139No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTCCTGGCAAGTAAAAAAAA3410.16569243402671485No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2360.1146727695903364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2330.11321506489215415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGG350.00356607862.127026145
AAGCAGT143600.061.154471
GTATCAA144500.060.6437239
GTGGTAT144900.060.290876
GGTATCA145700.060.2086268
TGGTATC146550.059.661537
AGTGGTA147000.059.4788935
CAGTGGT147500.059.404454
AGCAGTG147850.059.3619422
GCAGTGG149850.058.424443
CGTGCGG651.0922249E-555.755028145
CTGTCGA450.00963803848.321026145
TTGCGCC700.00105600741.41802145
AAATATA6250.040.58966145
CGGTCTG1105.2531104E-639.535385145
CTTGAGA2309.949872E-1031.513712145
ACCGGTT200.00607488528.999662120-124
AACCGAG200.006080657228.994024115-119
CTTGGGA4000.028.992615145
TACGCAC950.027.47336250-54
Produced by FastQC (version 0.11.5)