FastQCFastQC Report
Sun 19 Mar 2023
SRR4896098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856359
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG287583.3581710474228683No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84610.9880202111497631No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA38410.4485268444659307No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG34560.4035690639089447No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAATAGAAATTTATGTAG31880.37227377770304276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA29070.3394604365692426No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGTATTTTAATATTTGTT21820.25479968097491823No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTAGACGCAAACAAAATA18380.21462961211361123No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC16380.19127492091517692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA15490.18088208333187367No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGAAGAAATGCAAACTAT15040.17562727781222595No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTCTATGTTCATTCTATA14090.16453379949296965No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTTTAATATTTGTTATTCT13350.15589256374954896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTTAGTGTATATTTTGTT11570.13510688858294242No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA11530.13463979475897375No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGAAACTCCCTGTAAACT11440.1335888336550442No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGTTGAATGTGTATCTA9810.11455476032832025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT9740.11373734613637505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCACTGTATTTTAATATTT9480.1107012362805786No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTTGTTATTCTCTCATGAA9220.10766512642478213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT687900.0116.243991
GGTATCA707600.0113.26758
AGCAGTG705650.0113.206942
AGTGGTA704300.0113.108475
GTATCAA709150.0113.016969
GTGGTAT704950.0112.942466
TGGTATC706650.0112.7425847
CAGTGGT707900.0112.6525044
GCAGTGG716100.0111.352413
AGGGGTA3800.074.414015
GGGGTAT4850.056.8087926
GGGTATC5100.054.0240487
AGGTATC3850.050.8483287
TTGCGTA450.00963998748.33166145
GAGGTAT4650.042.1002356
CGGTCTG1953.6379788E-1240.89602145
CAGGGGT7150.039.5510144
AGCGGTA1859.640644E-1139.192415
AGAGGTA5200.037.6473245
GCGGTAT1951.70985E-1037.182546
Produced by FastQC (version 0.11.5)