FastQCFastQC Report
Sun 19 Mar 2023
SRR4896095_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896095_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674373
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT173982.5798779013987807No Hit
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT20900.30991750855980293No Hit
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT17030.25253086941499736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCA675600.0144.632482
CTGAGCC675950.0144.557591
GAGCCAT259550.0144.398123
GAGCCAG213300.0144.017443
AGCCATG115150.0143.57934
GCCAGAT21500.0143.552665
AGCCAGT75400.0143.459244
GAGCCAA192650.0143.245863
AGCCATA68950.0143.219334
AGCCAAT73050.0142.916994
AGCCAGA63550.0142.620474
AGCCATT75500.0141.92584
GCCATGT63900.0141.72595
GCCATTG27150.0141.164645
GCCATAG23250.0141.16125
GCCATAT22600.0141.053655
AGCCAGG75200.0140.565124
GCCAAGT17100.0140.240025
GCCAATG27350.0140.132355
AGCCAAG64850.0139.761474
Produced by FastQC (version 0.11.5)