FastQCFastQC Report
Sun 19 Mar 2023
SRR4896095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674373
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG365775.423852971575078No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG53880.7989643713493868No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA50050.7421708757616334No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34340.5092137437293605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC30270.4488613867992936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGGCGCGGTGGCTC13720.2034482400689233No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACAGGATCCGGAGCTGGT13100.19425451493461332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGCCAAGGCGAACCTAG9570.1419095960247519No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC8560.12693272120918245No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCACAGTGGCTCACG7820.11595956540371576No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA7630.11314213350771753No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG7600.11269727583992835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT303950.080.773351
AGCAGTG313150.078.371452
CAGTGGT332150.073.779194
GGTATCA333300.073.687778
GTGGTAT333450.073.371926
GTATCAA335300.073.231949
AGTGGTA336550.072.701485
TGGTATC337350.072.545197
GCAGTGG338400.072.416543
AAATATA25200.036.82378145
CGCTGTT2201.6370905E-1136.248405145
TTCGATA900.003628180832.220806145
GCACTTG5450.031.925201145
CCGGCGT2756.7593646E-926.362476145
AGGGGTA3108.54925E-1025.732315
GGGGTAT3108.54925E-1025.73046
TGCGGCT3751.0913936E-1125.132229145
AAACCGC11850.024.71621185-89
TGGTAGT2651.3755198E-724.62156145
ACGGTCT2951.4370016E-924.575191145
Produced by FastQC (version 0.11.5)