FastQCFastQC Report
Sun 19 Mar 2023
SRR4896091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94510
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG44334.69050894085282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG8830.9342926674425986No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA6150.6507247910274045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGGCGCGGTGGCTCAC5410.5724261982858957No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4880.5163474764575177No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGGGCGCGGTGGCTCACG4080.43170034916940003No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3370.3565760237011956No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC2230.23595386731562798No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1930.20421119458258383No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACATATCTGCTTCCGACACA1590.16823616548513387No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCATCTCCTCTAAC1370.1449582054809015No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1210.12802878002327794No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1140.12062215638556767No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATTGCCGGCGTCTTGGC1120.11850597820336473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTTATGAAAATGTTTTGCT1100.11638980002116178No Hit
AAGGAGTTCACCCCTGAAGTGCAGGCTGCCTGGCAGAAGCTGGTGTCTGC1100.11638980002116178No Hit
GTGATGGTGATTATTCTGGCTACTCACTTTGGCAAGGAGTTCACCCCTGA1090.11533171093006031No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTAAGTTATTAAGGGCGCAC1080.11427362183895884No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCTATAGACTTTTTTCAGAT1040.11004126547455295No Hit
TGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGT960.1015765527457412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCAA700.0103.54081145
CACAAAT353.4261822E-582.83265145
AGTGGTA41150.080.182765
AAGCAGT41650.079.8743441
GTGGTAT41350.079.794946
GGTATCA41550.079.759918
GTATCAA41550.079.7177059
CAGTGGT41450.079.6024254
AAATATA1650.079.06753145
AGCAGTG41750.079.030432
TGGTATC41950.078.8265157
GCAGTGG43850.075.080253
AATGGGG300.001932569172.47857145
GGTCTGA451.1900331E-464.4254145
TTCCGAG502.0038274E-457.982857145
TGTGGAA953.1061063E-853.405266145
AATAATG450.00960347248.3446272
AACAACC750.001474893338.67578
AAAATAT3150.034.513607145
GTATCAC950.00471270530.5172949
Produced by FastQC (version 0.11.5)