FastQCFastQC Report
Sun 19 Mar 2023
SRR4896070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404784
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG210515.200551405193881No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA29350.7250780663267323No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG27020.6675165026285624No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20320.501996126329104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACATATCTGCTTCCGACACA14830.36636823589865214No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC12690.31350053361792957No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9870.24383374836950078No Hit
GGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCC8560.21147080912289024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA7810.1929424087908613No Hit
CGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTG7380.18231945926716472No Hit
AAGCCCGCCTTTGCTAAGCTGAGTGAGCTGCACTGTGACAAGCTGCATGT7070.17466105379659275No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATATCTGCTTCCGACAC6980.17243764575674927No Hit
GCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAA6940.17144946440570774No Hit
TACTCACTTTGGCAAGGAGTTCACCCCTGAAGTGCAGGCTGCCTGGCAGA6520.16107356021977154No Hit
TGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGT6460.15959128819320922No Hit
CGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACA6250.15440333610024112No Hit
AAGGAGTTCACCCCTGAAGTGCAGGCTGCCTGGCAGAAGCTGGTGTCTGC6240.15415629076248075No Hit
GTGATGGTGATTATTCTGGCTACTCACTTTGGCAAGGAGTTCACCCCTGA6080.15020356535831456No Hit
TGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCAC5990.14798015731847108No Hit
GGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACG5970.1474860666429503No Hit
AACGTGATGGTGATTATTCTGGCTACTCACTTTGGCAAGGAGTTCACCCC5930.14649788529190877No Hit
GTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCA5560.1373572077947745No Hit
GCCTTTGCTAAGCTGAGTGAGCTGCACTGTGACAAGCTGCATGTGGATCC5340.131922210364046No Hit
CCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGT5280.1304399383374837No Hit
GATTATTCTGGCTACTCACTTTGGCAAGGAGTTCACCCCTGAAGTGCAGG5270.1301928929997233No Hit
GGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGG5220.12895766631092137No Hit
AACCTCAAGCCCGCCTTTGCTAAGCTGAGTGAGCTGCACTGTGACAAGCT5060.1250049409067552No Hit
CACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCC4970.12278153286691172No Hit
GACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCT4910.12129926084034942No Hit
TGAGAACTTCAAGCTCCTGGGTAACGTGATGGTGATTATTCTGGCTACTC4890.12080517016482865No Hit
GGTAACGTGATGGTGATTATTCTGGCTACTCACTTTGGCAAGGAGTTCAC4860.1200640341515475No Hit
ATGTGGATCCTGAGAACTTCAAGCTCCTGGGTAACGTGATGGTGATTATT4850.11981698881378709No Hit
GATGGTGATTATTCTGGCTACTCACTTTGGCAAGGAGTTCACCCCTGAAG4820.11907585280050595No Hit
TTCAAGCTCCTGGGTAACGTGATGGTGATTATTCTGGCTACTCACTTTGG4730.11685244476066248No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4690.11586426340962094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC4680.11561721807186054No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4660.11512312739633977No Hit
AACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCT4640.11462903672081899No Hit
CACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTT4590.11339381003201707No Hit
GAGAACTTCAAGCTCCTGGGTAACGTGATGGTGATTATTCTGGCTACTCA4460.11018222064113206No Hit
ACTTTGGCAAGGAGTTCACCCCTGAAGTGCAGGCTGCCTGGCAGAAGCTG4450.10993517530337167No Hit
ACCCCTGAAGTGCAGGCTGCCTGGCAGAAGCTGGTGTCTGCTGTCGCCAT4420.10919403929009051No Hit
CTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCT4410.10894699395233014No Hit
TAAGCTGAGTGAGCTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACT4370.10795881260128859No Hit
TAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTC4320.10672358591248667No Hit
TGAAGTGCAGGCTGCCTGGCAGAAGCTGGTGTCTGCTGTCGCCATTGCCC4290.1059824498992055No Hit
GTGATTATTCTGGCTACTCACTTTGGCAAGGAGTTCACCCCTGAAGTGCA4280.10573540456144512No Hit
ACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGTAAC4270.10548835922368474No Hit
TGAGTGAGCTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAG4250.10499426854816396No Hit
ACTCACTTTGGCAAGGAGTTCACCCCTGAAGTGCAGGCTGCCTGGCAGAA4200.10375904185936204No Hit
GGATCCTGAGAACTTCAAGCTCCTGGGTAACGTGATGGTGATTATTCTGG4180.10326495118384127No Hit
AAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGC4160.10277086050832049No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACTCGCTTCTGGAACG4120.10178267915727894No Hit
CAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACC4110.10153563381951856No Hit
CACTTTGGCAAGGAGTTCACCCCTGAAGTGCAGGCTGCCTGGCAGAAGCT4100.10128858848175816No Hit
TGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGTAACGT4100.10128858848175816No Hit
TGATTATTCTGGCTACTCACTTTGGCAAGGAGTTCACCCCTGAAGTGCAG4080.1007944978062374No Hit
TGCCTGGCAGAAGCTGGTGTCTGCTGTCGCCATTGCCCTGGCCCATAAGT4060.10030040713071664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATATA5950.084.07162145
AAGCAGT168450.069.11811
AGCAGTG172950.067.1184162
GGTATCA174350.066.9121558
GTATCAA174950.066.881649
GTGGTAT173650.066.639096
TGGTATC174000.066.588387
AGTGGTA174500.066.314485
CAGTGGT175250.066.15484
GCAGTGG183700.063.1512223
TGCGGAG2550.062.546036145
AGGGGTA606.7952424E-660.4212535
ACCCGGA606.7952424E-660.4212536
ACACGCG953.1312084E-853.4251026
CACGGGG450.00963684148.331028145
TGCGGCG951.9186627E-645.78729145
CCGGACA803.7290185E-545.3159378
AGAGAGT3550.040.84312145
CTACACG1252.7119677E-740.603084
TACACGC1252.7119677E-740.603085
Produced by FastQC (version 0.11.5)