FastQCFastQC Report
Sun 19 Mar 2023
SRR4896064_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896064_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445547
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCG100.0070999805144.928739
ACCGAGA446500.0144.58792
AACCGAG446550.0144.571721
CCGAGAT157100.0144.497363
CGAGAGT46200.0144.441964
CGAGATA48850.0144.170844
CCGAGAG148250.0143.934953
CCGAGAA135000.0143.89273
CGAGATG54600.0143.718124
CGAGAAG49150.0143.58574
CGAGAAT46600.0143.35764
CGAGATT53900.0142.627184
CGAGAGA48600.0142.07984
CGAGAGG53650.0141.266024
CGAGAAA40350.0137.190964
CGAGACT2050.0134.309114
GATATCG600.0132.836437
GAGATAC3450.0132.311375
GAGATTG20700.0129.511125
CCGAGAC8550.0127.963623
Produced by FastQC (version 0.11.5)