FastQCFastQC Report
Sun 19 Mar 2023
SRR4896048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771986
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC129580.7312698858794595No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA116520.6575672719761895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGTGGTGGCTCAT72090.40683165668351784No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59370.3350477938313282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGTGGTGGCTCATG49810.28109702898329897No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG46480.26230455545359843No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCACGGTGGCTCACG46270.26111944451028396No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG43930.24791392257049435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG42000.2370221886628901No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG41040.23160453863630978No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC36340.2050806270478435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCCCATATTTGATCTGT32380.18273282068819957No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC29530.16664917217178915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC27720.1564346445175075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC24310.13719070015225854No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGGTGCGGTGGCTCA23450.13233738867011363No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGGTGCGGTGGCTCAC22610.12759694489685583No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCCAGGCACAGTGGCTC22340.12607323082688013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAT21600.12189712559805778No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCATG21190.1195833375658724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA20970.1183417927681144No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCCGTGGCTCAC20700.11681807869813869No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCCGTGGCTCACG19990.11281127503264698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCCCATATTTGATCTGTC18440.10406402759389746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT541050.0135.946356
AAGCAGT542800.0135.464941
GGTATCA544600.0135.020518
TGGTATC545400.0134.901697
GTATCAA545850.0134.705739
AGTGGTA548700.0134.050985
CAGTGGT550950.0133.598794
AGCAGTG551900.0133.218022
GCAGTGG568900.0129.275133
CAACGCA540250.027.75084710-14
TCAACGC541050.027.6885210-14
GTACATG543250.027.51386820-24
AACGCAG546450.027.44389310-14
CGACCTA10600.027.44097745-49
ATCAACG544400.027.40968710-14
AGAGTAC546550.027.40865115-19
CAGAGTA547900.027.34111815-19
CGCAGAG547000.027.28869815-19
TACATGG547950.027.28312720-24
AGTACAT547200.027.22315820-24
Produced by FastQC (version 0.11.5)