FastQCFastQC Report
Sun 19 Mar 2023
SRR4896032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143721
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG185250.8641516316722185No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC111540.5203102455963252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT68970.32173029979181056No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55460.2587090390960391No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCAGTGGCTCA54770.2554903366622802No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCTCGGTGGCTCA53400.2490995796561213No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT52590.24532110288605652No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC44780.20888912316481484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC39270.18318615155610268No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC35140.1639205848149083No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG34700.16186807891512003No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG33530.1564102791361376No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG29960.13975699263103736No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC27160.12669559145056655No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA26870.12534280347116067No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC24790.11564004830852523No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC22350.1042579701369721No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA22190.10351160435523094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC22100.10309177360300151No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA21700.10122585914864855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT660300.0136.337051
GTGGTAT660550.0136.149676
GGTATCA661100.0136.03648
TGGTATC662050.0135.88477
GTATCAA665700.0135.070249
CAGTGGT671000.0134.125894
AGCAGTG671200.0134.021992
AGTGGTA675450.0133.178285
GCAGTGG702250.0128.157733
CAACGCA659200.027.80033910-14
TCAACGC659000.027.79566610-14
GTACATG660600.027.61588120-24
ATCAACG661600.027.60154310-14
AGAGTAC664300.027.51434515-19
CAGAGTA665550.027.47781415-19
TACATGG664700.027.45853820-24
AGTACAT664800.027.40025520-24
CGCAGAG669000.027.31243515-19
GCAGAGT670500.027.26207415-19
GAGTACA661350.027.14038820-24
Produced by FastQC (version 0.11.5)