FastQCFastQC Report
Sun 19 Mar 2023
SRR4896030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732040
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG52370.7153980656794711No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC34040.4650019124637998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23740.3242992186219332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG16330.2230752417900661No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT13170.17990820173760996No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA10990.15012840828370033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC10340.14124911207037866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG9920.1355117206710016No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA9050.12362712420086333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTCCGGCGGTGACGACC8670.11843615102999835No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC8590.11734331457297416No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC8330.11379159608764548No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCTTCTCGGCCTTTTGG7420.10136058138899515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT222700.0135.772741
GTGGTAT223500.0135.138646
GGTATCA223850.0134.95958
TGGTATC224750.0134.451117
GTATCAA225300.0134.132059
CAGTGGT226300.0133.48484
AGCAGTG226800.0133.190542
AGTGGTA228550.0132.121145
GCAGTGG229350.0131.615483
CGTGGTA1301.7346014E-533.23025
CAACGCA222250.027.82306910-14
TCAACGC222200.027.79692310-14
CGCGAAA2650.027.71273490-94
ATCAACG222550.027.63673610-14
GTACATG223450.027.59442520-24
AGAGTAC223650.027.57656515-19
TACATGG223750.027.57030120-24
CAGAGTA224750.027.4608215-19
CGCAGAG224850.027.35894615-19
AGTACAT225150.027.29653720-24
Produced by FastQC (version 0.11.5)