FastQCFastQC Report
Sun 19 Mar 2023
SRR4895971_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895971_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842186
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCTA1845250.0143.758072
ACAAGCT1845900.0143.707461
AAGCTAG678500.0143.607363
AGCTAGA186300.0143.072464
AAGCTAT551250.0142.863683
AGCTATG229850.0142.747014
AGCTAGT228800.0142.678314
AGCTATA157950.0142.632484
AAGCTAA499200.0142.543263
AGCTAGG252400.0142.402534
AGCTACG47300.0141.868934
AGCTAAT163400.0141.70874
GCTAGAT64800.0141.666675
GCTATGG93450.0141.226325
GCTAGTA64400.0141.204975
GCTAGTG87350.0140.95025
AAGCTAC127150.0140.94223
GCTAGGT88850.0140.434435
GCTAATG72400.0140.2215
GCTATGA59700.0140.02015
Produced by FastQC (version 0.11.5)