FastQCFastQC Report
Sun 19 Mar 2023
SRR4895966_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895966_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350148
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCT1355050.0142.030621
GCACCTC1355850.0141.946822
CACCTCG439850.0140.995743
ACCTCGT140500.0140.319244
ACCTCGA106850.0140.216644
CACCTCT396800.0139.336063
CACCTCA332950.0139.104783
ACCTCGG150850.0138.818664
ACCTCAG108250.0138.205694
ACCTCAT108500.0138.018634
ACCTCTA112550.0137.548454
ACCTCTG132200.0136.405044
CCTCGAT36800.0135.772435
CCTCGAG36600.0135.735385
CCTCGTA39450.0133.879035
CCTCTAG37200.0132.779715
ACCTCGC48050.0132.761154
CTCGAGT11800.0132.282826
CTCGATA11700.0132.195076
CACCTCC220000.0131.082463
Produced by FastQC (version 0.11.5)