FastQCFastQC Report
Sun 19 Mar 2023
SRR4895964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271918
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG134401.0566718923704201No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC82820.6511426051050461No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57780.4542745680146047No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA48620.38225734677864454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC39230.30843183286972903No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATGCAAATGTTTTGAAA38880.3056800831500144No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTTTTTCCAAATGATCCTA36070.283587463971734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA31400.24687126056868444No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAGCAAAAAAAAAAAAAAA24020.18884865219298727No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCACAAAAAAAAAAAAA23630.18578241679101953No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA23180.18224445286567215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAGCCCTGGACTCATCAT22670.1782347604169451No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAACAAGCATGATCTTGT21700.1706084826223074No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGATCTTGTTACTGTGATA21660.17029399694005432No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTGGGTGATTATTTTTT17130.1346784934248906No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAGCATATGCAGCCAA15960.1254797872189874No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACTGTTTTAATAAAAGATT14000.11006998878858544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC13560.10661064628380132No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAATGATCCTAGTAATTGCC13510.10621753918098494No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAATGAAGTGATTCTAAGAT13500.10613891776042166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA13110.10307268235845392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT782600.0141.836531
GGTATCA795900.0139.672218
GTATCAA797850.0139.31289
GTGGTAT797900.0139.195796
AGCAGTG798100.0139.04582
TGGTATC799850.0138.964467
CAGTGGT799900.0138.892764
AGTGGTA801200.0138.613485
GCAGTGG803300.0138.163653
CAACGCA795800.028.57126210-14
TCAACGC795350.028.5602710-14
AGAGTAC792150.028.54294215-19
ATCAACG795500.028.53859310-14
CAGAGTA793850.028.48363515-19
TACATGG790700.028.4691520-24
GTACATG791400.028.46398520-24
CGCAGAG797300.028.42720815-19
AGTACAT792400.028.4117120-24
GCAGAGT795400.028.3756315-19
AACGCAG801800.028.32872610-14
Produced by FastQC (version 0.11.5)