FastQCFastQC Report
Sun 19 Mar 2023
SRR4895910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804702
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG61160.7600329065915085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC46980.5838186061423981No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC34960.4344465404584555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC32660.40586453121776755No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG26610.33068141995421907No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14590.18130935427027645No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA11680.1451468991005366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT10690.1328442081665014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA10560.13122870329637554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC10520.1307316248747984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC10290.1278734239507296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT9850.12240556131338061No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC9390.11668915946524304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205800.0138.010648
AAGCAGT205950.0137.533361
GTGGTAT207300.0136.838366
GTATCAA208350.0136.42529
TGGTATC208800.0136.027747
AGCAGTG209950.0134.844452
AGTGGTA210500.0134.758165
CAGTGGT211150.0134.445624
GCAGTGG212950.0133.249153
CAACGCA203850.028.51673310-14
TCAACGC205600.028.30202310-14
GTACATG204400.028.25014120-24
ATCAACG206550.028.16487910-14
AGTACAT204850.028.15293520-24
TACATGG206100.028.03808220-24
AGAGTAC206550.027.99163615-19
CGCAGAG207100.027.93120415-19
CAGAGTA207700.027.87825215-19
AACGCAG209100.027.77320110-14
GCAGAGT209150.027.68497515-19
Produced by FastQC (version 0.11.5)