FastQCFastQC Report
Sun 19 Mar 2023
SRR4895733_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895733_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307050
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATACCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT122570.9377606059446845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCA1310900.0143.776821
TACCAAG447050.0143.410083
ATACCAA1314650.0143.366712
TACCAAT476150.0143.049513
ACCAAGT159500.0142.256584
ACCAATG182850.0141.992174
ACCAATA141050.0140.059884
TACCAAA380550.0139.937423
CCAATGT82000.0139.716055
CCAATAG49550.0138.379935
ACCAAGA133000.0138.128754
ACCAATT157550.0138.068054
ACCAAGG163200.0137.882744
CCAATTG58000.0137.238945
CCAAGAT48400.0137.198875
CAATGTA46900.0137.128686
CCAATGG55200.0136.493975
CCAAGTG54050.0136.063225
CCAATGA44250.0136.053425
CCAAATG52650.0134.203545
Produced by FastQC (version 0.11.5)