FastQCFastQC Report
Sat 18 Mar 2023
SRR4895638_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895638_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742146
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34830.46931466315253334No Hit
ATCCTGTAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT7570.102001492967691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGTA743750.0143.90012
ATCCTGT744200.0143.81311
CCTGTAG236600.0143.61053
CCTGTAT268200.0143.33283
CCTGTAA212700.0143.270783
CTGTATG96900.0142.806144
CTGTAGT94750.0142.774054
CTGTAAG68800.0142.647894
CTGTAAT80800.0142.256674
CTGTATA76100.0141.756364
CTGTAGG78050.0141.633134
CTGTAGA64700.0141.545174
CTGTATT96350.0139.729554
CTGTAAA64550.0138.969594
CTGTACT21100.0134.99274
CCTGTAC30750.0134.370863
CTGTACG5250.0133.231864
TGTAGTA27200.0130.964525
TGTAATG31900.0130.4315
TGTAGTG32500.0129.798055
Produced by FastQC (version 0.11.5)