FastQCFastQC Report
Sat 18 Mar 2023
SRR4895634_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895634_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455834
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG100.00721203144.1728
AGAGTCA456200.0144.04561
GAGTCAA456500.0143.950932
AGTCAAT167300.0143.48263
AGTCAAG157150.0143.438083
GTCAAGT55450.0143.131994
GTCAATG61950.0142.892034
AGTCAAA126950.0142.411733
GTCAAAT46900.0142.327594
GTCAATT56650.0142.136054
GTCAATA48650.0141.949424
GTCAAAG43000.0141.657384
GTCAAGA47700.0141.60294
GTCAAGG54350.0140.98884
TCAAGTG17600.0137.618735
TCAAGAG15900.0137.371435
TCAATGG20900.0137.273825
TCAAATG17450.0137.149295
TCAATAG16100.0137.008185
TCAATGA19250.0136.682565
Produced by FastQC (version 0.11.5)