FastQCFastQC Report
Sat 18 Mar 2023
SRR4895584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438826
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24580.5601308947054185No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC13330.30376504582681974No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9160.2087387711758191No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC6050.13786785650804648No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5010.11416825803393602No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT4920.11211733124290721No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT4590.10459726634246831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT70550.0136.403431
GGTATCA70750.0135.61098
GTGGTAT71250.0134.659246
GTATCAA71450.0134.28239
TGGTATC71700.0133.814127
AGTGGTA71800.0133.627735
CAGTGGT72200.0132.987094
AGCAGTG72350.0132.910352
GCAGTGG74850.0128.374973
TCAACGC70900.027.69421410-14
ATCAACG70700.027.6911110-14
AGAGTAC71250.027.43694915-19
GTACATG71100.027.41884420-24
TACATGG71250.027.38132720-24
CAGAGTA71450.027.36014715-19
CAACGCA72300.027.21768210-14
AGTACAT71500.027.2050420-24
GCAGAGT73000.026.83837115-19
GAGTACA71250.026.7548920-24
CGCAGAG73200.026.7257115-19
Produced by FastQC (version 0.11.5)