FastQCFastQC Report
Sat 18 Mar 2023
SRR4895581_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895581_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087622
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCT1089850.0143.579391
TTGGCTC1092100.0143.283582
TGGCTCA248550.0143.18183
TGGCTCG284250.0142.990653
TGGCTCT334000.0142.605653
GGCTCGA71500.0141.763554
GGCTCAG75200.0141.580524
GGCTCTA88450.0141.273794
GGCTCAT84550.0141.068654
TGGCTCC231350.0140.984593
GGCTCGT94800.0140.689294
GCTCGAT25550.0140.497735
GCTCGTA25250.0140.172675
GGCTCTG109750.0140.009144
GCTCATG27400.0139.41315
GCTCAGT28050.0138.747155
GCTCGAG20950.0138.725715
GCTCTAG27850.0138.452015
GCTCATA26250.0138.121375
GGCTCGG90950.0137.865084
Produced by FastQC (version 0.11.5)