FastQCFastQC Report
Sat 18 Mar 2023
SRR4895567_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895567_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386591
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATC1389750.0143.592362
AAGAGAT1392450.0143.313951
GAGATCA337900.0143.199683
GAGATCG413300.0143.157653
GAGATCT434900.0142.633123
AGATCGA104850.0142.619744
GAGATCC210400.0142.453063
AGATCGT152100.0141.983964
AGATCTA114650.0141.852464
AGATCTG132750.0141.701224
AGATCAG103900.0141.638174
GATCGAT42400.0140.699075
AGATCCG53500.0140.561054
AGATCAT117650.0140.559634
AGATCAA92200.0140.411534
GATCGTA46000.0140.169245
GATCGAG30000.0140.085825
GATCATG39050.0139.685295
AGATCGG119300.0139.098164
AGATCGC43350.0138.94374
Produced by FastQC (version 0.11.5)