FastQCFastQC Report
Sat 18 Mar 2023
SRR4895532_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895532_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3470962
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211840.6103207122405835No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC75210.2166834439558831No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGTGGTGGCTCA65350.18827633376568226No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT59290.17081719707677584No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC58790.16937667424765815No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA57290.16505510576030508No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA49270.14194911958125728No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA45920.13229761662616876No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT45690.13163497612477462No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT44740.12889798274945102No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA44170.12725578672425686No Hit
GTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAG43980.12670838804919213No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG43390.12500857111083324No Hit
GGCAGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCG42990.1238561528475391No Hit
CACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAG40850.11769071513891538No Hit
CGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGG39970.11515539495966824No Hit
GCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGT39450.11365725121738585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG39250.11308104208573877No Hit
TGCTCACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCC38460.11080501601573281No Hit
CCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTAC38190.11002713368800925No Hit
CAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGT37860.10907638862079158No Hit
AGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGC37700.10861542131547393No Hit
GTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCC37440.10786634944433274No Hit
AGACGCTTTCAGTGGGTTGACGGCAGCCGCTGGAACTTTGCGTACTGGGC37400.10775110761800331No Hit
GAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTC37110.10691560437711504No Hit
CGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGG37010.1066274998112915No Hit
ACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGA37000.10659868935470915No Hit
AGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTC36650.10559032337432678No Hit
AGGATCACAGGCTCGGGTCGCTGCAGACGCTTTCAGTGGGTTGACGGCAG36450.1050141142426797No Hit
AGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAG36200.10429385282812084No Hit
CGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCAT36110.10403455871887968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA407800.0137.253028
GTGGTAT409250.0136.749136
AAGCAGT411000.0136.536711
GTATCAA412300.0136.174099
TGGTATC413350.0135.410147
AGTGGTA415850.0134.596085
CAGTGGT419550.0133.460564
AGCAGTG419600.0133.377942
GCAGTGG425650.0131.465263
AGAGTAC407100.028.01085315-19
TCAACGC408600.027.99244310-14
ATCAACG409250.027.9268710-14
TACATGG405650.027.87693220-24
CAGAGTA410000.027.77409415-19
GTACATG408600.027.71090520-24
AGTACAT412250.027.5563820-24
CAACGCA416500.027.54792810-14
GAGTACA406600.027.38681220-24
GCAGAGT417700.027.18625515-19
TATCAAC414500.027.10763710-14
Produced by FastQC (version 0.11.5)