FastQCFastQC Report
Sat 18 Mar 2023
SRR4895515_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895515_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880971
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCTA2891900.0143.45022
GTTCTAG971750.0143.298973
GTTCTAA845750.0143.178193
GTTCTAT1079250.0142.827973
GTGTTCT2917400.0142.196331
TTCTAGG342300.0141.324164
TTCTAGA270800.0140.432754
TCTAATG111600.0140.318125
TCTAGTA109300.0139.911385
TCTAGGT132000.0139.850085
TCTATGG131700.0139.7865
CTAGGTA41650.0139.500986
TTCTATG379200.0139.43864
TCTAGGA96150.0139.427695
TTCTAAG292450.0139.391714
TCTATGA108550.0139.286215
TTCTAGT376850.0139.219134
TCTATAG116800.0138.940785
TCTAGAG88500.0138.788825
TCTAGAT100600.0138.341525
Produced by FastQC (version 0.11.5)