FastQCFastQC Report
Sat 18 Mar 2023
SRR4895514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332722
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98770.741114801136321No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG54510.40901253224603484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC36490.27380053754646505No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA19130.14354081346297276No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC17950.13468675387665247No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA14990.11247657050757773No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT14560.10925009116679997No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC14500.10879988474715657No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT14280.10714912787513076No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA13830.1037725797278052No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA13350.10017092837065794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169700.0135.668038
AAGCAGT170650.0135.025851
GTGGTAT170650.0134.912776
TGGTATC170750.0134.833767
GTATCAA172500.0133.465889
AGCAGTG175400.0131.451342
CAGTGGT175800.0131.001484
AGTGGTA175750.0130.874955
GCAGTGG179400.0128.279593
CGTGGTA1054.0233335E-641.1251035
TCAACGC169700.027.90545710-14
AGAGTAC169300.027.86936415-19
ATCAACG169400.027.83591810-14
CAGAGTA170750.027.6242715-19
CAACGCA171750.027.6142810-14
TACATGG170800.027.56277720-24
GTACATG171500.027.45027520-24
AGTACAT171650.027.3256420-24
GCAGAGT174150.027.09321615-19
CGCAGAG174250.027.07766515-19
Produced by FastQC (version 0.11.5)