FastQCFastQC Report
Sat 18 Mar 2023
SRR4895500_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895500_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506340
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31600.624086582138484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG19360.3823517794367421No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC13360.26385432713196666No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA7080.13982699371963503No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA5460.10783268159734567No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC5240.10348777501283722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT101300.0138.33481
GTGGTAT101850.0137.80256
TGGTATC102650.0136.868797
GGTATCA102550.0136.861888
GTATCAA103000.0136.194069
AGTGGTA103350.0135.732835
AGCAGTG103700.0135.133222
CAGTGGT104000.0135.022924
GCAGTGG106050.0132.206653
CAACGCA102000.028.211510-14
TCAACGC102100.028.1556710-14
AGAGTAC102200.028.11403515-19
ATCAACG102000.028.1127110-14
CGCAGAG102350.027.9743815-19
GTACATG102300.027.9648820-24
CAGAGTA102600.027.9623415-19
AACGCAG103250.027.9257210-14
AGTACAT102750.027.74432420-24
TACATGG103100.027.73392720-24
GCAGAGT103450.027.70475415-19
Produced by FastQC (version 0.11.5)