FastQCFastQC Report
Sat 18 Mar 2023
SRR6792050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6792050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100479798
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG18554741.8466139830416457No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA11192811.1139363556443456No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG9278860.9234552800354953No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7960610.7922597535476734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAATCCTAGCCCTAGCCC6744670.6712463733257107No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA4887050.4863713997514207No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA4779160.475633917974238No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA3601960.35847603913375703No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGTGAGATGGCTCAGT2794970.2781623824522418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2542670.2530528574510072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG1795740.1787165217031985No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG1466760.1459756119334555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA1187130.11814613719665319No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA1151480.11459816031875382No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTC1042490.10375120379919553No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGAGAGATGGCTCA1028410.10234992709678815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT56326650.0125.584691
AGTGGTA56508750.0124.8894045
GTGGTAT56656000.0124.515936
GTATCAA56907700.0124.2007459
GGTATCA56850200.0124.181648
TGGTATC56904050.0124.007187
AGCAGTG57118450.0123.672572
GCAGTGG57215300.0123.316513
CAGTGGT57352450.0123.063064
TAGTACC1029500.098.389786145
CCCTCTT3139700.052.19441145
CCTCTTC2070450.040.019493145
TGCGGAT232200.035.0407145
CATGGGG16787450.026.29493325-29
ATGGGGG7075600.025.94325325-29
ACCCGAC2503150.025.33758560-64
AGTACAT56470600.025.22228420-24
ATGGGAT7841500.025.20952625-29
TACATGG56088150.025.20369120-24
GTACATG56392500.025.18411620-24
Produced by FastQC (version 0.11.5)